Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-EL-R2856
文献（863）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 15.63-1000 pg/mL
灵敏度： 9.38 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.38 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

The protective role of neuregulin-1: A potential therapy for sepsis-induced cardiomyopathy

IF: 2.730

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2016)

DOI：10.1016/j.ejphar.2016.06.042

PMID：27346832

Reactivity：Rat

Sample Type：Serum
Necrostatin-1 protects against oleic acid-induced acute respiratory distress syndrome in rats

IF: 2.371

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2016)

DOI：10.1016/j.bbrc.2016.08.163

PMID：27586277

Reactivity：Rat

Sample Type：Bronchoalveolar Lavage Fluid
SIRT1 exerts neuroprotective effects by attenuating cerebral ischemia/reperfusion-induced injury via targeting p53/microRNA-22

IF: 2.348

Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2016)

DOI：10.3892/ijmm.2016.2806

PMID：27878231

Reactivity：Rat

Sample Type：Tissue homogenate
LncRNA NONRATT021972 siRNA rescued decreased heart rate variability in diabetic rats in superior cervical ganglia

IF: 1.621

Journal： AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL (2016)

DOI：10.1016/j.autneu.2016.07.012

PMID：27519467

Reactivity：Rat

Sample Type：serum
Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study

IF: 1.617

Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2016)

DOI：10.3109/08923973.2016.1173058

PMID：27144896

Reactivity：Rat

Sample Type：Serum
Effect of apelin hormone on renal ischemia/reperfusion induced oxidative damage in rats

IF: 0.875

Journal： RENAL FAILURE (2016)

DOI：10.1080/0886022X.2016.1184957

PMID：27197832

Reactivity：Rat

Sample Type：Serum
Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats

IF: 0.580

Journal： Acta Cirurgica Brasileira (2016)

DOI：10.1590/S0102-865020160070000004

PMID：27487279

Reactivity：Rat

Sample Type：Serum
Role of Rho Kinase Inhibition in the Protective Effect of Fasudil and Simvastatin Against 3-Nitropropionic Acid-Induced Striatal Neurodegeneration and Mitochondrial Dysfunction in Rats

IF: 5.137

Journal： MOLECULAR NEUROBIOLOGY (2015)

DOI：10.1007/s12035-015-9303-2

PMID：26169112

Reactivity：Rat

Sample Type：the second striatum
Polydatin Alleviates Small Intestine Injury during Hemorrhagic Shock as a SIRT1 Activator

IF: 3.516

Journal： Oxidative Medicine and Cellular Longevity (2015)

DOI：10.1155/2015/965961

PMID：26301045

Reactivity：Rat

Sample Type：Serum
Transplantation of Induced Pluripotent Stem Cells Alleviates Cerebral Inflammation and Neural Damage in Hemorrhagic Stroke

IF: 3.234

Journal： PLoS One (2015)

DOI：10.1371/journal.pone.0129881

PMID：26086994

Reactivity：Rat

Sample Type：Tissue homogenate

Species

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Canine

Human

Mouse

Porcine

Rat

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