Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit
中文名称:大鼠心肌肌钙蛋白I(TNNI3/cTn-I)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNNI3/cTn-I抗体包被于酶标板上,实验时样品(或标准品)中的大鼠TNNI3/cTn-I会与包被抗体结合。后依次加入生物素化的抗大鼠TNNI3/cTn-I抗体和辣根过氧化物酶标记的亲和素,抗大鼠TNNI3/cTn-I抗体与结合在包被抗体上的大鼠TNNI3/cTn-I结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNNI3/cTn-I浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNNI3/cTn-I的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠TNNI3/cTn-I,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
操作步骤 | ![]() |
数据处理 |
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Euterpe oleracea extract (a?aí) exhibits cardioprotective effects after chemotherapy treatment in a breast cancer model
IF: 3.9Journal: BMC Complementary Medicine and Therapies (2023)PMID:37626388Reactivity:RatSample Type:cardiac tissue -
Dexmedetomidine postconditioning attenuates myocardial ischemia/reperfusion injury by activating the Nrf2/Sirt3/SOD2 signaling pathway in the rats
IF: 3.800 -
Protective effect of canagliflozin on post-resuscitation myocardial function in a rat model of cardiac arrest
IF: 3.5Journal: Intensive Care Medicine Experimental (2023)PMID:37966667Reactivity:RatSample Type:myocardial tissue -
Lipopolysaccharides protect mesenchymal stem cell against cardiac ischemia-reperfusion injury by HMGB1/STAT3 signaling
IF: 2.5 -
Melatonin attenuates inflammation and cardiac dysfunction in myocardial infarction by regulating the miRNA-200b-3p/high mobility group box chromosomal protein 1 axis
IF: 2.2Journal: JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)Reactivity:RatSample Type:serum -
Nrf2 protects against myocardial ischemia-reperfusion injury in diabetic rats by inhibiting Drp1-mediated mitochondrial fission
IF: 2.1 -
SMOC2 plays a role in heart failure via regulating TGF-β1/Smad3 pathway-mediated autophagy
IF: 2.1 -
Vagus Nerve Stimulation Attenuates Septic Shock-Induced Cardiac Injury in Rats
IF: 2.1Journal: PHYSIOLOGICAL RESEARCH (2023)Reactivity:RatSample Type:serum -
The key mediators involved in myocardial endoplasmic reticulum stress induced by ischaemia reperfusion injury in rats
IF: 0.700Journal: European Journal of Inflammation (2023)Reactivity:RatSample Type:serum -
Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia?reperfusion injury
IF: 9.429

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