Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit
中文名称：大鼠心肌肌钙蛋白I(TNNI3/cTn-I)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNNI3/cTn-I抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNNI3/cTn-I会与包被抗体结合。后依次加入生物素化的抗大鼠TNNI3/cTn-I抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNNI3/cTn-I抗体与结合在包被抗体上的大鼠TNNI3/cTn-I结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNNI3/cTn-I浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNNI3/cTn-I的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠TNNI3/cTn-I，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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Cardioprotective effect of allyl isothiocyanate in a rat model of doxorubicin acute toxicity
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Dynamic observation of 5?fluorouracil?induced myocardial injury and mitochondrial autophagy in aging rats
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34721693Reactivity：RatSample Type：serum -
Carbon monoxide attenuates LPS-induced myocardial dysfunction in rats by regulating the mitochondrial dynamic equilibrium
IF: 3.263 -
Treatment with 17β-estradiol protects donor heart against brain death effects in female rat
IF: 3.177 -
The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572927Reactivity：RatSample Type：Serum -
Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase?1/IL?1β Signaling Pathway Inhibition
IF: 2.511Journal： IMMUNOLOGICAL INVESTIGATIONS (2020)PMID：32674625Reactivity：RatSample Type：Cell culture supernatant -
Evaluation of 2 Rat Models for Sepsis Developed by Improved Cecal Ligation/Puncture or Feces Intraperitoneal-Injection
IF: 1.918 -
St. Thomas and del Nido cardioplegia are superior to Custodiol cardioplegia in a rat model of donor heart
IF: 1.084Journal： SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020)PMID：33185130Reactivity：RatSample Type：Tissue homogenate -
Sepsis causes heart injury through endoplasmic reticulum stress-mediated apoptosis signaling pathway.
IF: 0.252Journal： International Journal of Clinical and Experimental Pathology (2020)DOI：PMID:32509067PMID：32509067Reactivity：RatSample Type：serum -
Exogenous NADPH ameliorates myocardial ischemia–reperfusion injury in rats through activating AMPK/mTOR pathway
IF: 4.010
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