Rat ACE2(Angiotensin Ⅰ Converting Enzyme 2) ELISA Kit
中文名称：大鼠血管紧张素转化酶2(ACE2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ACE2抗体包被于酶标板上，实验时样品（或标准品）中的大鼠ACE2会与包被抗体结合。后依次加入生物素化的抗大鼠ACE2抗体和辣根过氧化物酶标记的亲和素，抗大鼠ACE2抗体与结合在包被抗体上的大鼠ACE2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ACE2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ACE2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ACE2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
N-Methyl-d-Aspartate-Induced Excitotoxicity and Its Impact on the Renin–Angiotensin System in Retinal Tissue
IF: 1.900Journal： JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS (2024)Reactivity：RatSample Type：retinal tissue -
Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
IF: 0.700Journal： Natural Products Journal (2024)Reactivity：RatSample Type：Serum -
Rutin protects against?gamma-irradiation and malathion-induced oxidative stress and inflammation?through regulation of mir-129-3p, mir-200C-3p, and mir-210 gene expressions in rats’ kidney
IF: 5.800Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)PMID：37184799Reactivity：RatSample Type：kidney -
Caution may be required in using l-theanine in diabetes mellitus: A study on the rats
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37199135Reactivity：RatSample Type：Kidney,heart -
COVID-19: Understanding the impact of anti-hypertensive drugs and hydroxychloroquine on the ACE1 and ACE2 in lung and adipose tissue in SHR and WKY rats
IF: 2.500 -
Pioglitazone Alters Ace/Ace 2 Balance to Favour Ace2 Independently Of Glycaemia Levels in Diabetic Rat Heart
IF: 1.600 -
Angiotensin 1–7 mitigates rhabdomyolysis induced renal injury in rats via modulation of TLR-4/NF-kB/iNOS and Nrf-2/heme?oxygenase-1 signaling pathways
IF: 6.780 -
Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system
IF: 2.107 -
Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats
IF: 1.522Journal： CLINICAL AND EXPERIMENTAL HYPERTENSION (2019)PMID：30648895Reactivity：RatSample Type：Serum -
Spironolactone rescues renal dysfunction in obstructive jaundice rats by upregulating ACE2 expression
IF: 2.516Journal： Journal of Cell Communication and Signaling (2018)PMID：29882088Reactivity：RatSample Type：Tissue homogenate
实验操作视频
