Rat ACE(Angiotensin Ⅰ Converting Enzyme) ELISA Kit
中文名称：大鼠血管紧张素转化酶(ACE)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ACE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠ACE会与包被抗体结合。后依次加入生物素化的抗大鼠ACE抗体和辣根过氧化物酶标记的亲和素，抗大鼠ACE抗体与结合在包被抗体上的大鼠ACE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ACE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ACE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ACE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Optimization of Ultrasound Extraction of Total Anthocyanin From Berberis kaschgarica Rupr. by Response Surface Methodology and Its Antihypertensive Effect
IF: 3.500N-Methyl-d-Aspartate-Induced Excitotoxicity and Its Impact on the Renin–Angiotensin System in Retinal Tissue
IF: 1.900Journal： JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS (2024)Reactivity：RatSample Type：retinal tissueEffects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
IF: 0.700Journal： Natural Products Journal (2024)Reactivity：RatSample Type：SerumRutin protects against?gamma-irradiation and malathion-induced oxidative stress and inflammation?through regulation of mir-129-3p, mir-200C-3p, and mir-210 gene expressions in rats’ kidney
IF: 5.800Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)PMID：37184799Reactivity：RatSample Type：kidney2-phenylacetamide Separated from the seed of Lepidium apetalum Willd. inhibited renal fibrosis via MAPK pathway mediated RAAS and oxidative stress in SHR Rats
IF: 3.900Journal： BMC Complementary Medicine and Therapies (2023)PMID：37353787Reactivity：RatSample Type：serum,renalBuchholzia coriacea seed induce antifertility by interfering with steroidogenic enzymes and inflammatory cytokines in rat testis
IF: 3.400Journal： JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023)PMID：36868158Reactivity：RatSample Type：TesticularCOVID-19: Understanding the impact of anti-hypertensive drugs and hydroxychloroquine on the ACE1 and ACE2 in lung and adipose tissue in SHR and WKY rats
IF: 2.500Pioglitazone Alters Ace/Ace 2 Balance to Favour Ace2 Independently Of Glycaemia Levels in Diabetic Rat Heart
IF: 1.600Kolaviron attenuates cardiovascular injury in fructose-streptozotocin induced type-2 diabetic male rats by reducing oxidative stress, inflammation, and improving cardiovascular risk markers
IF: 6.530Centella asiatica (L.) Urb. Prevents Hypertension and Protects the Heart in Chronic Nitric Oxide Deficiency Rat Model
IF: 5.811
