Rat AChE(Acetylcholinesterase) ELISA Kit
中文名称：大鼠乙酰胆碱酯酶(AChE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠AChE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠AChE会与包被抗体结合。后依次加入生物素化的抗大鼠AChE抗体和辣根过氧化物酶标记的亲和素，抗大鼠AChE抗体与结合在包被抗体上的大鼠AChE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，AChE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中AChE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠AChE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Moderate capsaicin-containing kochujang alleviates memory impairment through the gut-brain axis in rats with scopolamine-induced amnesia
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：hippocampi tissue -
Evaluation of terpenes rich Hura crepitans extract on glucose regulation and diabetic complications in STZ-induced diabetic rats
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：brain tissue -
Protective Effect of the Novel Melatonin Analogue Containing Donepezil Fragment on Memory Impairment via MT/ERK/CREB Signaling in the Hippocampus in a Rat Model of Pinealectomy and Subsequent Aβ1-42 Infusion
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)PMID：38339146Reactivity：RatSample Type：hippocampus -
Efficacy and Mechanism of Schisandra chinensis Fructus Water Extract in Alzheimer’s Disease: Insights from Network Pharmacology and Validation in an Amyloid-β Infused Animal Model
IF: 4.800 -
Neuroprotective effect of ranolazine improves behavioral discrepancies in a rat model of scopolamine-induced dementia
IF: 4.300 -
Metformin effectively alleviates the symptoms of Alzheimer in rats by lowering amyloid β deposition and enhancing the insulin signal
IF: 3.200Journal： METABOLIC BRAIN DISEASE (2024)Reactivity：RatSample Type：hippocampus tissue -
Effects of quercetin-immobilized albumin cerium oxide nanoparticles on glutamate toxicity: in vitro study
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：Rat -
Silica Nanoparticles from Melon Seed Husk Abrogated Binary Metal(loid) Mediated Cerebellar Dysfunction by Attenuation of Oxido-inflammatory Response and Upregulation of Neurotrophic Factors in Male Albino Rats
IF: 2.700Journal： CEREBELLUM (2024)Reactivity：RatSample Type：cerebellar tissue -
Role of bone marrow mesenchymal stem cell-derived exosomes in reducing neurotoxicity and depression-like behaviors induced by doxorubicin in rats
IF: 2.200Journal： Toxicology Research (2024)Reactivity：RatSample Type：brain tissue -
Role of vitamin D3 in mitigating sodium arsenite-induced neurotoxicity in male rats
IF: 2.200Journal： Toxicology Research (2024)Reactivity：RatSample Type：cerebral cortex tissue
实验操作视频
