Rat AChE(Acetylcholinesterase) ELISA Kit
中文名称：大鼠乙酰胆碱酯酶(AChE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠AChE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠AChE会与包被抗体结合。后依次加入生物素化的抗大鼠AChE抗体和辣根过氧化物酶标记的亲和素，抗大鼠AChE抗体与结合在包被抗体上的大鼠AChE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，AChE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中AChE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠AChE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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The protective role of l-carnitine on oxidative stress, neurotransmitter perturbations, astrogliosis, and apoptosis induced by thiamethoxam in the brains of male rats
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：38099937Reactivity：RatSample Type：brain tissue -
Memory-Improving Activity of the Flower Extract from Chrysanthemum boreale (Makino) Maskino in Scopolamine-Treated Rodents
IF: 3.500 -
Ni and Al mixture amplifies cerebellar oxido-inflammatory responses, down regulates AChE and BDNF/NGF levels in motor impairment in male albino rats
IF: 3.500Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2023)PMID：37864919Reactivity：RatSample Type：brain -
Nickel and aluminium mixture elicit memory impairment by activation of oxidative stress, COX-2, and diminution of AChE, BDNF and NGF levels in cerebral cortex and hippocampus of male albino rats
IF: 3.300Journal： Current Research in Toxicology (2023)PMID：37841055Reactivity：RatSample Type：hippocampus,frontal cortex -
Alzheimer's disease-related brain insulin resistance and the prospective therapeutic impact of metformin
IF: 3.200Journal： JOURNAL OF NEUROENDOCRINOLOGY (2023)PMID：37985011Reactivity：RatSample Type：hippocampus tissue -
Coconut oil ameliorates behavioral and biochemical alterations induced by D-GAL/AlCl3 in rats
IF: 2.900 -
N-acetylcysteine ameliorates chemotherapy-induced impaired anxiety and depression-like behaviors by regulating inflammation, oxidative and cholinergic status, and BDNF release.
IF: 2.700Journal： BEHAVIOURAL BRAIN RESEARCH (2023)PMID：37926333Reactivity：RatSample Type：Hippocampal tissue -
The effect of the calcium channel blocker nimodipine on hippocampal BDNF/Ach levels in rats with experimental cognitive impairment
IF: 1.900Journal： NEUROLOGICAL RESEARCH (2023)PMID：36598971Reactivity：RatSample Type：Hippocampal,prefrontal cortex -
Effect of bisphenol F on sexual performance and quality of offspring in Male Wistar rats
IF: 7.129Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)PMID：36115153Reactivity：RatSample Type：plasma -
Integrated comparative metabolomics and network pharmacology approach to uncover the key active ingredients of Polygonati rhizoma and their therapeutic potential for the treatment of Alzheimer's disease.
IF: 5.988
实验操作视频
