Rat AChE(Acetylcholinesterase) ELISA Kit
中文名称：大鼠乙酰胆碱酯酶(AChE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠AChE抗体包被于酶标板上，实验时样品（或标准品）中的大鼠AChE会与包被抗体结合。后依次加入生物素化的抗大鼠AChE抗体和辣根过氧化物酶标记的亲和素，抗大鼠AChE抗体与结合在包被抗体上的大鼠AChE结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，AChE浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中AChE的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠AChE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Protective Role of Acetate Against Depressive-Like Behaviour Associated with Letrozole-Induced PCOS Rat Model: Involvement of HDAC2 and DNA Methylation
IF: 5.682Journal： MOLECULAR NEUROBIOLOGY (2022)PMID：36269541Reactivity：RatSample Type：PFC, hippocampal tissues -
Modulation of High-Fat Diet-Induced Brain Oxidative Stress by Ferulate-Rich Germinated Brown Rice Ethyl Acetate Extract
IF: 4.927 -
Differential effects of voluntary and forced exercise trainings on spatial learning ability and hippocampal biomarkers in aged female rats
IF: 3.197 -
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains
IF: 2.693Journal： Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022)DOI：10.1002/jez.2603PMID：35546266Reactivity：RatSample Type：brain -
Alteration of the α5 GABA receptor and 5HTT lead to cognitive deficits associated with major depressive-like behaviors in a 14-day combined stress rat model
IF: 2.590Journal： INTERNATIONAL JOURNAL OF NEUROSCIENCE (2022)PMID：34937496Reactivity：RatSample Type：hippocampus,Prefrontal cortex(PFC) -
Nootropic effect of Syzygium polyanthum (Wight) Walp?leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia
Journal： Advances in Traditional Medicine (2022)Reactivity：RatSample Type：brain -
Naproxen as a potential candidate for promoting rivastigmine anti-Alzheimer activity against aluminum chloride-prompted Alzheimer's-like disease in rats; neurogenesis and apoptosis modulation as a possible underlying mechanism
IF: 4.432Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2021)PMID：34914971Reactivity：RatSample Type：hippocampal tissue -
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades
IF: 3.647 -
Possible combined effect of perindopril and Azilsartan?in an experimental model of dementia in rats
IF: 2.879Journal： SAUDI PHARMACEUTICAL JOURNAL (2020)PMID：32435138Reactivity：RatSample Type：Tissue homogenate -
Protective Effects of Centella asiatica on Cognitive Deficits Induced by D-gal/AlCl3 via Inhibition of Oxidative Stress and Attenuation of Acetylcholinesterase Level
实验操作视频
