Rat ACTH(Adrenocorticotropic Hormone) ELISA Kit
中文名称：大鼠促肾上腺皮质激素(ACTH)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠ACTH抗原包被于酶标板上，实验时样品或标准品中的大鼠ACTH与包被的大鼠ACTH竞争生物素标记的抗大鼠ACTH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠ACTH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠ACTH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ACTH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Exploring the mechanism of Paotianxiong polysaccharide in the treatment of chronic kidney disease combining metabolomics and microbiomics technologies
IF: 7.700Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)Reactivity：RatSample Type：serumLactobacillus reuteri or Lactobacillus rhamnosus GG intervention facilitates gut barrier function, decreases corticosterone and ameliorates social behavior in LPS-exposed offspring
IF: 7.000Journal： FOOD RESEARCH INTERNATIONAL (2024)Reactivity：RatSample Type：SerumPregestational Prediabetes Induces Maternal Hypothalamic–Pituitary–Adrenal (HPA) Axis Dysregulation and Results in Adverse Foetal Outcomes
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：PlasmaPromoting wound healing using Cyanothece sp. in the rat model
IF: 2.800Journal： JOURNAL OF APPLIED PHYCOLOGY (2024)Reactivity：RatSample Type：plasmaEffects of combined postweaning social isolation and ketamine administration on schizophrenia-like behaviour in male Sprague Dawley rats
IF: 2.600Journal： BEHAVIOURAL BRAIN RESEARCH (2024)Reactivity：RatSample Type：PlasmaHyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization
IF: 2.400Journal： NEUROSCIENCE RESEARCH (2024)Reactivity：RatSample Type：blood sampleAcupuncture exerts preventive effects in rats of chronic unpredictable mild stress: The involvement of inflammation in amygdala and brain-spleen axis
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：36706710Reactivity：RatSample Type：Serum,amygdalaAcupuncture Alleviates CUMS-Induced Depression-Like Behaviors by Restoring Prefrontal Cortex Neuroplasticity
IF: 3.100Electroacupuncture in the treatment of IBS in rats: Investigation of the mechanisms of CRH+ neurons in the paraventricular nucleus
IF: 2.500Acute exposure to microcystins affects hypothalamic-pituitary axes of male rats
IF: 9.988
