Rat AMH(Anti-Mullerian Hormone) ELISA Kit
中文名称：大鼠抗缪勒管激素(AMH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠AMH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠AMH会与包被抗体结合。后依次加入生物素化的抗大鼠AMH抗体和辣根过氧化物酶标记的亲和素，抗大鼠AMH抗体与结合在包被抗体上的大鼠AMH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，AMH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中AMH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠AMH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145–5p
IF: 3.400 -
Hydrogen-rich water treatment targets RT1-Db1 and RT1-Bb to alleviate premature ovarian failure in rats
IF: 2.700 -
HGF-modified human umbilical cord mesenchymal stem cells rescue impaired ovarian reserve function in chemotherapy-induced POI rats by improving angiogenesis while decreasing apoptosis and fibrosis in the ovary
IF: 2.600 -
Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota
IF: 10.723 -
Increased phosphatase regenerating liver-1 trigger vascular remodeling in injured ovary via platelet-derived growth factor signaling pathway
IF: 8.079 -
miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failure
IF: 8.079 -
Arsenic exposure diminishes ovarian follicular reserve and induces abnormal steroidogenesis by DNA methylation
IF: 7.129Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)PMID：36068745Reactivity：RatSample Type：Serum -
Moxibustion mitigates mitochondrial dysfunction and NLRP3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats
IF: 6.780 -
Can a Large Number of Transplanted Mesenchymal Stem Cells Have an Optimal Therapeutic Effect on Improving Ovarian Function?
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36555651Reactivity：RatSample Type：serum -
Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone
IF: 3.251
实验操作视频
