Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
中文名称：大鼠血管紧张素Ⅱ(Ang-Ⅱ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Ang-Ⅱ抗原包被于酶标板上，实验时样品或标准品中的大鼠Ang-Ⅱ与包被的大鼠Ang-Ⅱ竞争生物素标记的抗大鼠Ang-Ⅱ单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠Ang-Ⅱ浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠Ang-Ⅱ的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Ang-Ⅱ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Optimization of Ultrasound Extraction of Total Anthocyanin From Berberis kaschgarica Rupr. by Response Surface Methodology and Its Antihypertensive Effect
IF: 3.500 -
Bile Acid Metabolism Analysis Provides Insights into Vascular Endothelial Injury in Salt-Sensitive Hypertensive Rats
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Study on material basis and anti-hypertensive metabolomics of Zhengan-Xifeng-Tang(ZXT): A comparison between ZXT decoction and granules
IF: 3.000Journal： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2024)PMID：38447242Reactivity：RatSample Type：serum -
Losartan is more effective than angiotensin (1–7) in preventing thyroxine-induced renal injury in the rat
IF: 1.900 -
Renal Denervation Ameliorates Cardiomyocyte Apoptosis in Myocardial Ischemia–Reperfusion Injury Through Regulating Mitochondria–Endoplasmic Reticulum Contact
IF: 1.400Journal： Anatolian Journal of Cardiology (2024)Reactivity：RatSample Type：Serum -
Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
IF: 0.700Journal： Natural Products Journal (2024)Reactivity：RatSample Type：Serum -
Antihypertensive activity of different components of Veratrum alkaloids through metabonomic data analysis
IF: 7.900 -
Wnt/β-catenin signaling pathway promotes abnormal activation of fibroblast-like synoviocytes and angiogenesis in rheumatoid arthritis and the intervention of Er Miao San
IF: 7.900 -
Chronic arsenite exposure induced skeletal muscle atrophy by disrupting angiotensin II-melatonin axis in rats
IF: 4.500 -
2-phenylacetamide Separated from the seed of Lepidium apetalum Willd. inhibited renal fibrosis via MAPK pathway mediated RAAS and oxidative stress in SHR Rats
IF: 3.900Journal： BMC Complementary Medicine and Therapies (2023)PMID：37353787Reactivity：RatSample Type：serum,renal
实验操作视频
