Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
中文名称：大鼠血管紧张素Ⅱ(Ang-Ⅱ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Ang-Ⅱ抗原包被于酶标板上，实验时样品或标准品中的大鼠Ang-Ⅱ与包被的大鼠Ang-Ⅱ竞争生物素标记的抗大鼠Ang-Ⅱ单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠Ang-Ⅱ浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠Ang-Ⅱ的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Ang-Ⅱ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Perindopril Dampens Cd-induced Nephrotoxicity by Suppressing Inflammatory Burden, Ang II/Ang 1–7, and Apoptosis Signaling Pathways
IF: 3.900 -
Study on material basis and anti-hypertensive metabolomics of different extraction methods of the Uncaria rhynchophylla Scrophularia Formula
IF: 3.400Journal： JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2023)PMID：37209496Reactivity：RatSample Type：serum -
Balancing renal Ang-II/Ang-(1–7) by xanthenone; an ACE2 activator; contributes to the attenuation of Ang-II/p38 MAPK/NF-κB p65 and Bax/caspase-3 pathways in amphotericin B-induced nephrotoxicity in rats
IF: 3.200 -
Effects of a high-salt diet on MAP and expression levels of renal ENaCs and aquaporins in SHR
IF: 3.200Journal： EXPERIMENTAL BIOLOGY AND MEDICINE (2023)PMID：37828834PMC10792424Reactivity：RatSample Type：plasma -
Acacetin Combined with Wortmannin Activated Insulin Signaling Pathway by Inhibiting the Induction Effect of PI3Kp110 on IRS-1 Serine Phosphorylation to Improve Myocardial Fibrosis in Fructose-Induced SHR Rats
IF: 1.800Journal： Natural Product Communications (2023)Reactivity：RatSample Type：serum -
Controlled Hemorrhage Sensitizes Angiotensin II-Elicited Hypertension through Activation of the Brain Renin-Angiotensin System Independently of Endoplasmic Reticulum Stress
IF: 7.310Journal： Oxidative Medicine and Cellular Longevity (2022)PMID：35069977Reactivity：RatSample Type：plasma -
The association between obesity and vitamin D deficiency modifies the progression of kidney disease after ischemia/reperfusion injury
IF: 6.590Journal： Frontiers in Nutrition (2022)Reactivity：RatSample Type：blood,renal tissue -
Azithromycin Mitigates Cisplatin-Induced Lung Oxidative Stress, Inflammation and Necroptosis by Upregulating SIRT1, PPARγ, and Nrf2/HO-1 Signaling
IF: 5.215 -
Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.
IF: 5.058 -
Activation of angiotensin-converting enzyme 2 ameliorates metabolic syndrome-induced renal damage in rats by renal TLR4 and nuclear transcription factor κB downregulation
IF: 5.058Journal： Frontiers in Medicine (2022)Reactivity：RatSample Type：renal tissue
实验操作视频
