Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
中文名称：大鼠血管紧张素Ⅱ(Ang-Ⅱ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Ang-Ⅱ抗原包被于酶标板上，实验时样品或标准品中的大鼠Ang-Ⅱ与包被的大鼠Ang-Ⅱ竞争生物素标记的抗大鼠Ang-Ⅱ单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠Ang-Ⅱ浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠Ang-Ⅱ的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Ang-Ⅱ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction
IF: 2.287Journal： International Journal of Endocrinology (2019)PMID：31396276Reactivity：RatSample Type：serum -
Hyperuricemia Inhibition Protects SD Rats Against Fructose-Induced Obesity Hypertension Via Modulation of Inflammation and Renin-Angiotensin System in Adipose Tissue
IF: 1.927Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2019)PMID：31683330Reactivity：RatSample Type：Serum -
Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats
IF: 1.522Journal： CLINICAL AND EXPERIMENTAL HYPERTENSION (2019)PMID：30648895Reactivity：RatSample Type：Serum -
COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism
IF: 4.358 -
Metformin attenuates cardiovascular and renal injury in uninephrectomized rats on DOCA-salt: Involvement of AMPK and miRNAs in cardioprotection
IF: 3.616 -
The probable roles of valsartan in alleviating chronic obstructive pulmonary disease following co-exposure to cold stress and fine particulate matter
IF: 2.776Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2018)PMID：29775776Reactivity：RatSample Type：Tissue homogenate -
Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling
IF: 1.694Journal： BIOLOGICAL & PHARMACEUTICAL BULLETIN (2018)PMID：30068867Reactivity：RatSample Type：Plasma -
Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin?angiotensin system activity
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402152Reactivity：RatSample Type：Plasma,Tissue homogenate -
Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy
IF: 4.085 -
Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS
IF: 2.861
实验操作视频
