Rat ANP(Atrial Natriuretic Peptide) ELISA Kit
中文名称：大鼠心钠肽(ANP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠ANP抗原包被于酶标板上，实验时样品或标准品中的大鼠ANP与包被的大鼠ANP竞争生物素标记的抗大鼠ANP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠ANP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠ANP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ANP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Targeting the adenosine monophosphate-activated protein kinase signalling pathway by bempedoic acid attenuates Angiotensin II-induced cardiac remodelling in renovascular hypertension in rats
IF: 6.100 -
Linagliptin and secoisolariciresinol diglucoside attenuate hyperlipidemia and cardiac hypertrophy induced by a high-methionine diet in rats via suppression of hyperhomocysteinemia-induced endoplasmic reticulum stress
IF: 5.600 -
Vanillic Acid Alleviates Right Ventricular Function in Rats With MCT-Induced Pulmonary Arterial Hypertension
IF: 1.800Journal： Natural Product Communications (2023)Reactivity：RatSample Type：serum -
Natriuretic Peptides—New Targets for Neurocontrol of Blood Pressure via Baroreflex Afferent Pathway
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36362405Reactivity：RatSample Type：nucleus tractus solitarius (NTS) tissue -
Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway
IF: 6.530 -
Differential effects of speed and volume on transfusion-associated circulatory overload: A randomized study in rats
IF: 2.144 -
Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators
IF: 5.546Journal： JOURNAL OF CELLULAR PHYSIOLOGY (2020)PMID：31960959Reactivity：RatSample Type：Cell culture supernatant,Tissue homogenate -
Exendin?4 inhibits atrial arrhythmogenesis in a model of myocardial infarction?induced heart failure via the GLP?1 receptor signaling pathway
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32855719Reactivity：RatSample Type：Plasma -
Synergistic effect of Aconiti Lateralis Radix Praeparata water-soluble alkaloids and Ginseng Radix et Rhizoma total ginsenosides compatibility on acute heart failure rats
IF: 2.813Journal： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2019)PMID：31877430Reactivity：RatSample Type：Serum -
High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid
IF: 2.229Journal： FUNDAMENTAL & CLINICAL PHARMACOLOGY (2019)PMID：30739350Reactivity：RatSample Type：Cell culture medium
实验操作视频
