Rat BALP(Bone Alkaline Phosphatase) ELISA Kit
中文名称：大鼠骨碱性磷酸酶(BALP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠BALP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠BALP会与包被抗体结合。后依次加入生物素化的抗大鼠BALP抗体和辣根过氧化物酶标记的亲和素，抗大鼠BALP抗体与结合在包被抗体上的大鼠BALP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，BALP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中BALP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠BALP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Isoflavones isolated from chickpea sprouts alleviate ovariectomy-induced osteoporosis in rats by dual regulation of bone remodeling
IF: 7.500 -
Evaluation of terpenes rich Hura crepitans extract on glucose regulation and diabetic complications in STZ-induced diabetic rats
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：serum -
Bovine lactopontin promotes bone development in growing rats by altering the composition of intestinal flora and bile acid metabolism
IF: 5.100 -
Multiscale effects of the calcimimetic drug, etelcalcetide on bone health of rats with secondary hyperparathyroidism induced by chronic kidney disease
IF: 3.500 -
Role of cacao (Theobroma cacao L.) extracts in alveolar bone remodeling in diabetic rats during orthodontic tooth movements
IF: 1.400Journal： Journal of Advanced Pharmaceutical Technology & Research (2024)Reactivity：RatSample Type：gingival crevicular fluid -
Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats
IF: 0.700Journal： Natural Products Journal (2024)Reactivity：RatSample Type：Serum -
Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis
IF: 8.900 -
Active components and mechanisms of total flavonoids from Rhizoma Drynariae in enhancing cranial bone regeneration: An investigation employing serum pharmacochemistry and network pharmacology approaches
IF: 5.400 -
Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling
IF: 3.925 -
Roxadustat promotes osteoblast differentiation and prevents estrogen deficiency-induced bone loss by stabilizing HIF-1α and activating the Wnt/β-catenin signaling pathway
IF: 2.677Journal： Journal of Orthopaedic Surgery and Research (2022)PMID：35597989Reactivity：RatSample Type：serum
实验操作视频
