Rat bFGF/FGF2(Basic Fibroblast Growth Factor) ELISA Kit
中文名称：大鼠碱性成纤维细胞生长因子(bFGF/FGF2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠bFGF/FGF2抗体包被于酶标板上，实验时样品（或标准品）中的大鼠bFGF/FGF2会与包被抗体结合。后依次加入生物素化的抗大鼠bFGF/FGF2抗体和辣根过氧化物酶标记的亲和素，抗大鼠bFGF/FGF2抗体与结合在包被抗体上的大鼠bFGF/FGF2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，bFGF/FGF2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中bFGF/FGF2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠bFGF/FGF2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Q1:E-EL-R0091 Rat bFGF/FGF2，如果bFGF失活了，ELISA还能测出来不？
失活样本的蛋白构象已发生改变，有可能影响抗原抗体结合，因此我们无法保证ELISA检测结果，建议客户使用新鲜收集样本进行检测。
实验操作视频
