Rat BNP(Brain Natriuretic Peptide) ELISA Kit
中文名称：大鼠脑钠素(BNP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠BNP抗原包被于酶标板上，实验时样品或标准品中的大鼠BNP与包被的大鼠BNP竞争生物素标记的抗大鼠BNP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠BNP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠BNP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠BNP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis
IF: 40.800Journal： Signal Transduction and Targeted Therapy (2024)Reactivity：RatSample Type：heart tissue -
The isoproterenol-induced myocardial fibrosis: A biochemical and histological investigation
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：serum -
The redox-active defensive Selenoprotein T as a novel stress sensor protein playing a key role in the pathophysiology of heart failure
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：RatSample Type：plasma -
Cardioprotective Effect of Hydroalcohol Extract of Andaliman (Zanthoxylum acanthopodium DC.) Fruits on Doxorubicin-Induced Rats
IF: 4.600 -
Pretreatment with Indole-3-Propionic Acid Attenuates Lipopolysaccharide-Induced Cardiac Dysfunction and Inflammation Through the AhR/NF-κB/NLRP3 Pathway
IF: 4.200Journal： Journal of Inflammation Research (2024)Reactivity：RatSample Type：serum -
Palliative potential of velutin against abamectin induced cardiac toxicity via regulating JAK1/STAT3, NF-κB, Nrf-2/Keap-1 signaling pathways: An insight from molecular docking
IF: 4.200Journal： PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2024)Reactivity：RatSample Type：cardiac tissue -
Paeoniflorin attenuates cuproptosis and ameliorates left ventricular remodeling after AMI in hypobaric hypoxia environments
IF: 3.300Journal： Journal of Natural Medicines (2024)PMID：38427210Reactivity：RatSample Type：serum,cardiac tissue -
Discovery of a novel 1,3,4-oxadiazol-2-one-based NLRP3 inhibitor as a pharmacological agent to mitigate cardiac and metabolic complications in an experimental model of diet-induced metaflammation
IF: 6.700Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2023)PMID：37290185Reactivity：RatSample Type：plasma -
Echinacoside ameliorates doxorubicin?induced cardiac injury by regulating GPX4 inhibition?induced ferroptosis
IF: 2.700Journal： Experimental and Therapeutic Medicine (2023)Reactivity：RatSample Type：heart tissue -
Exosomes secreted from bone marrow mesenchymal stem cells suppress cardiomyocyte hypertrophy through Hippo-YAP pathway in heart failure
IF: 2.100
实验操作视频
