Rat BNP(Brain Natriuretic Peptide) ELISA Kit
中文名称：大鼠脑钠素(BNP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠BNP抗原包被于酶标板上，实验时样品或标准品中的大鼠BNP与包被的大鼠BNP竞争生物素标记的抗大鼠BNP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠BNP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠BNP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠BNP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Vanillic Acid Alleviates Right Ventricular Function in Rats With MCT-Induced Pulmonary Arterial Hypertension
IF: 1.800Journal： Natural Product Communications (2023)Reactivity：RatSample Type：serum -
Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway
IF: 6.780 -
LCZ696 Protects against Diabetic Cardiomyopathy-Induced Myocardial Inflammation, ER Stress, and Apoptosis through Inhibiting AGEs/NF-κB and PERK/CHOP Signaling Pathways
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：35163209Reactivity：RatSample Type：serum -
Natriuretic Peptides—New Targets for Neurocontrol of Blood Pressure via Baroreflex Afferent Pathway
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36362405Reactivity：RatSample Type：nucleus tractus solitarius (NTS) tissue -
The mechanism of Renshen-Fuzi herb pair for treating heart failure—Integrating a cardiovascular pharmacological assessment with serum metabolomics
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：RatSample Type：serum -
Identification of potential biomarkers of inflammation-related genes for ischemic cardiomyopathy
IF: 5.846 -
Exosomes derived from human umbilical cord mesenchymal stem cells (HUCMSC-EXO) regulate autophagy through AMPK-ULK1 signaling pathway to ameliorate diabetic cardiomyopathy
IF: 3.322Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022)PMID：36240643Reactivity：RatSample Type：serum -
Parkia speciosa Hassk. Empty Pod Extract Prevents Cardiomyocyte Hypertrophy by Inhibiting MAPK and Calcineurin-NFATC3 Signaling Pathways
IF: 3.251 -
Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy
IF: 2.751Journal： Experimental and Therapeutic Medicine (2022)PMID：35493422Reactivity：RatSample Type：serum -
Rutin Modulates MAPK Pathway Differently from Quercetin in Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy
IF: 5.924Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID：34064664Reactivity：RatSample Type：Cell lysate
实验操作视频
