Rat BNP(Brain Natriuretic Peptide) ELISA Kit
中文名称：大鼠脑钠素(BNP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠BNP抗原包被于酶标板上，实验时样品或标准品中的大鼠BNP与包被的大鼠BNP竞争生物素标记的抗大鼠BNP单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠BNP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠BNP的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠BNP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 5.811 -
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Empagliflozin Significantly Prevents the Doxorubicin-induced Acute Cardiotoxicity via Non-antioxidant Pathways
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Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：33603819Reactivity：RatSample Type：Serum -
Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy
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WY14643 improves left ventricular myocardial mitochondrial and systolic functions in obese rats under chronic persistent hypoxia via the PPARα pathway
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Plasma MIR-212-3p as a biomarker for acute right heart failure with pulmonary artery hypertension
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Carbon monoxide attenuates LPS-induced myocardial dysfunction in rats by regulating the mitochondrial dynamic equilibrium
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The effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration extract on X-irradiation-induced myocardial fibrosis in rats
IF: 3.743 -
Therapeutic effects of erythropoietin expressed in mesenchymal stem cells for dilated cardiomyopathy in rat
IF: 2.705Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2019)PMID：31400858Reactivity：RatSample Type：Serum,Tissue homogenate
实验操作视频
