Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Indole-3-propionic acid mitigates chlorpyrifos-mediated neurotoxicity by modulating cholinergic and redox-regulatory systems, inflammatory stress, apoptotic responses and DNA damage in rats
IF: 4.860Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021)PMID：34915193Reactivity：RatSample Type：brain -
Effects of catechin on a rodent model of autism spectrum disorder: implications for the role of nitric oxide in neuroinflammatory pathway
IF: 4.530 -
Selenium abates manganese–induced striatal and hippocampal toxicity via abrogation of neurobehavioral deficits, biometal accumulation, oxidative stress, inflammation, and caspase-3 activation in rats
IF: 4.530 -
Neuroprotective effects of roflumilast against quinolinic acid-induced rat model of Huntington’s disease through inhibition of NF-κB mediated neuroinflammatory markers and activation of cAMP/CREB/BDNF signaling pathway
IF: 4.473Journal： INFLAMMOPHARMACOLOGY (2021)PMID：33517508Reactivity：RatSample Type：Tissue homogenate(brain) -
Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage
IF: 4.219 -
Conivaptan and Boric Acid Treatments in Acute Kidney Injury: Is This Combination Effective and Safe?
IF: 3.738 -
Biochemical and histological alterations of doxorubicin-induced neurotoxicity in rats: Protective role of luteolin
IF: 3.652Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021)PMID：34766659Reactivity：RatSample Type：brain -
Guggulsterone ameliorates ethidium bromide-induced experimental model of multiple sclerosis via restoration of behavioral, molecular, neurochemical and morphological alterations in rat brain
IF: 3.584Journal： METABOLIC BRAIN DISEASE (2021)PMID：33635478Reactivity：RatSample Type：Tissue homogenate(brain) -
Chlorogenic acid abates oxido-inflammatory and apoptotic responses in the liver and kidney of Tamoxifen-treated rats
IF: 3.524 -
Manganese mitigates against hepatorenal oxidative stress, inflammation and caspase-3 activation in rats exposed to hexachlorobenzene
IF: 3.356Journal： DRUG AND CHEMICAL TOXICOLOGY (2021)Reactivity：RatSample Type：Liver tissue,kidney tissue
实验操作视频
