Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Co-administration of Luteolin mitigated toxicity in rats' lungs associated with doxorubicin treatment
IF: 3.347Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2020)PMID：33358696Reactivity：RatSample Type：Tissue homogenate -
Comparative effects of galactose-induced aging on mitochondrial permeability transition in rat liver and testis
IF: 2.295 -
Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions
IF: 0.373Journal： Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020)Reactivity：RatSample Type：serum -
In vivo exposure to codeine induces reproductive toxicity: role of HER2 and p53/Bcl-2 signaling pathway
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Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic
IF: 5.714 -
Impact of binary waterborne mixtures of nickel and zinc on hypothalamic-pituitary-testicular axis in rats
IF: 5.108 -
Safranal, a constituent of saffron, exerts gastro-protective effects against indomethacin-induced gastric ulcer
IF: 3.448 -
The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats
IF: 3.361Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)PMID：31767546Reactivity：RatSample Type：Tissue homogenate -
Neuroprotective mechanisms of selenium against arsenic-induced behavioral impairments in rats
IF: 3.263 -
Dietary protocatechuic acid abrogates male reproductive dysfunction in streptozotocin-induced diabetic rats via suppression of oxidative damage, inflammation and caspase-3 activity
IF: 3.170Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2019)PMID：30690002Reactivity：RatSample Type：Tissue homogenate
实验操作视频
