Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Neurobehavioural and biochemical responses associated with exposure to binary waterborne mixtures of zinc and nickel in rats
IF: 3.061Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2019)PMID：31734518Reactivity：RatSample Type：cerebellum, cerebrum, liver -
Fluoride and diethylnitrosamine coexposure enhances oxido-inflammatory responses and caspase-3 activation in liver and kidney of adult rats
IF: 2.965Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)PMID：30920066Reactivity：RatSample Type：Serum -
Co-treatment of piracetam with risperidone rescued extinction deficits in experimental paradigms of post-traumatic stress disorder by restoring the physiological alterations in cortex and hippocampus
IF: 2.773Journal： PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2019)PMID：31445955Reactivity：RatSample Type：plasma -
Zinc abrogates anticancer drug tamoxifen-induced hepatotoxicity by suppressing redox imbalance, NO/iNOS/NF-?B signaling, and caspase-3-dependent apoptosis in female rats
IF: 2.276 -
Hepatorenal protective effects of protocatechuic acid in rats administered with anticancer drug methotrexate.
IF: 2.171Journal： HUMAN & EXPERIMENTAL TOXICOLOGY (2019)PMID：31431087Reactivity：RatSample Type：hepatic and renal -
Behavioral, histopathological, and biochemical evaluations on the effects of cinnamaldehyde, naloxone, and their combination in morphine-induced cerebellar toxicity
IF: 1.946Journal： DRUG AND CHEMICAL TOXICOLOGY (2019)PMID：31656103Reactivity：RatSample Type：Tissue homogenate -
Crocin exerts improving effects on indomethacin-induced small intestinal ulcer by antioxidant, anti-inflammatory and anti-apoptotic mechanisms
IF: 0.723Journal： Veterinary Research Forum (2019)PMID：32206222Reactivity：RatSample Type：Small intestine tissue -
Protocatechuic acid ameliorates neurobehavioral deficits via suppression of oxidative damage, inflammation, caspase-3 and acetylcholinesterase activities in diabetic rats
IF: 3.977Journal： FOOD AND CHEMICAL TOXICOLOGY (2018)PMID：30597223Reactivity：RatSample Type：Tissue homogenate -
The effects of safranal, a constitute of saffron, and metformin on spatial learning and memory impairments in type-1 diabetic rats: behavioral and hippocampal histopathological and biochemical evaluations
IF: 3.457Journal： BIOMEDICINE & PHARMACOTHERAPY (2018)PMID：30092399Reactivity：RatSample Type：Tissue homogenate -
Protective effects of L-theanine against doxorubicin-induced nephrotoxicity in rats
IF: 3.457Journal： BIOMEDICINE & PHARMACOTHERAPY (2018)PMID：30372854Reactivity：RatSample Type：plasma,renal tissues
实验操作视频
