Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Acetate attenuates lead-induced dysregulation of testicular steroidogenesis and spermatogenesis by targeting oxidative stress, inflammatory cytokines, and apoptosis
IF: 1.600Journal： Toxicological Research (2024)Reactivity：RatSample Type：testicular tissue -
Lansoprazole induces hippocampal cell apoptosis and impairs learning and memory functions via activation of tau phosphorylation and Aβ (1–42) pathway in adult rats
IF: 1.600Journal： Toxicology and Environmental Health Sciences (2024)Reactivity：RatSample Type：hippocampus tissue -
Acetate attenuates cyclophosphamide-induced cardiac injury via inhibition of NF-kB signaling and suppression of caspase 3-dependent apoptosis in Wistar rats
IF: 7.500Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)PMID：38128178Reactivity：RatSample Type：cardiac tissue -
Protective effect of arbutin against cyclophosphamide-induced oxidative stress, inflammation, and hepatotoxicity via Nrf2/HO-1 pathway in rats
IF: 5.800Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)PMID：37119491Reactivity：RatSample Type：liver -
A combination of metformin and epigallocatechin gallate potentiates glioma chemotherapy in vivo
IF: 5.600Journal： Frontiers in Pharmacology (2023)PMID：37025487Reactivity：RatSample Type：C6 cell-Xenograft -
Omega-3 fatty acid ameliorates bisphenol F-induced testicular toxicity by modulating Nrf2/NFkB pathway and apoptotic signaling
IF: 5.200Journal： Frontiers in Endocrinology (2023)PMID：37800144Reactivity：RatSample Type：testicular tissues -
Protective effect of eicosapentaenoic acid against estradiol valerate-induced endometrial hyperplasia via modulation of NF-κB/HIF-1α/VEGF signaling pathway in rats
IF: 5.100Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2023)PMID：36774993Reactivity：RatSample Type：uterine tissue -
Effects of Tiliroside and Lisuride Co-Treatment on the PI3K/Akt Signal Pathway: Modulating Neuroinflammation and Apoptosis in Parkinson’s Disease
IF: 4.700 -
The Hydrophobic Extract of Sorghum bicolor (L. Moench) Enriched in Apigenin-Protected Rats against Aflatoxin B1-Associated Hepatorenal Derangement
IF: 4.600 -
Dietary aflatoxin B1 and antimalarial—a lumefantrine/artesunate—therapy perturbs male rat reproductive function via pro-inflammatory and oxidative mechanisms
IF: 4.600Journal： Scientific Reports (2023)PMID：37500724Reactivity：RatSample Type：hypothalamic,epididymal,testicular
实验操作视频
