Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Exogenous taurine administration abates reproductive dysfunction in male rats exposed to silver nanoparticles
IF: 4.500Journal： ENVIRONMENTAL TOXICOLOGY (2023)PMID：37638810Reactivity：RatSample Type：hypothalamus,epididymis,testes -
Selenium and zinc alleviate hepatotoxicity induced by heavy metal mixture (cadmium, mercury, lead and arsenic) via attenuation of inflammo-oxidant pathways
IF: 4.500 -
Interleukin-35 and Thymoquinone Nanoparticle-Based Intervention for Liver Protection against Paracetamol-Induced Liver Injury in Rats
IF: 4.400Journal： SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2023)PMID：37766887Reactivity：RatSample Type：hepatic tissues -
Purmorphamine, a Smo-Shh/Gli Activator, Promotes Sonic Hedgehog-Mediated Neurogenesis and Restores Behavioural and Neurochemical Deficits in Experimental Model of Multiple Sclerosis
IF: 4.400 -
SNORC knockdown alleviates inflammation, autophagy defect and matrix degradation of chondrocytes in osteoarthritis development
IF: 4.300 -
Acetate Abates Arsenic-Induced Male Reproductive Toxicity by Suppressing HDAC and Uric Acid–Driven Oxido-inflammatory NFkB/iNOS/NO Response in Rats
IF: 3.900Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2023)PMID：37726447Reactivity：RatSample Type：testicular tissues -
Restraint Stress Exacerbates Apoptosis in a 6-OHDA Animal Model of Parkinson Disease
IF: 3.700 -
Amelioration of neurobehavioral, biochemical, and morphological alterations associated with silver nanoparticles exposure by taurine in rats
IF: 3.600Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2023)PMID：37437208Reactivity：RatSample Type：cerebrum,cerebellum -
Sodium acetate ameliorates doxorubicin-induced cardiac injury via upregulation of Nrf2/HO-1 signaling and downregulation of NFkB-mediated apoptotic signaling in Wistar rats
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37458777Reactivity：RatSample Type：cardiac tissue -
Modulatory effect of ciprofloxacin, a broad spectrum antibacterial drug, on mPT pore using rat model with estradiol benzoate–induced endometrial hyperplasia
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37943297Reactivity：RatSample Type：liver tissue
实验操作视频
