Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Proton Pump Dysfunction and Upregulation of Caspase-3 Activity via Oxidative-Sensitive Signaling Mediate Rohypnol-Induced Testicular Toxicity
IF: 2.400 -
Zinc and selenium attenuate quaternary heavy metal mixture-induced testicular damage via amplification of the antioxidant system, reduction in metal accumulation, inflammatory and apoptotic biomarkers
IF: 2.300 -
Selenium and zinc protect against heavy metal mixture-induced, olfactory bulb and hippocampal damage by augmenting antioxidant capacity and activation of Nrf2-Hmox-1 signaling in male rats
IF: 2.200Journal： INTERNATIONAL JOURNAL OF NEUROSCIENCE (2023)PMID：38108304Reactivity：RatSample Type：olfactory bulb (OB),Hippocampus (HIP) -
Naringenin Prevents Renal Injury in Experimental Hyperuricemia Through Suppressing Xanthine Oxidase, Inflammation, Apoptotic Pathway, DNA Damage, and Activating Antioxidant System
IF: 2.100Journal： Metabolic Syndrome and Related Disorders (2023)PMID：37195720Reactivity：RatSample Type：kidney -
Selenium and zinc supplementation mitigates metals-(loids) mixture- mediated cardiopulmonary toxicity via attenuation of antioxidant, anti-inflammatory and antiapoptotic mechanisms in female Sprague Dawley rats
IF: 2.100Journal： Toxicology Research (2023)PMID：38179003PMC10762678Reactivity：RatSample Type：heart tissue,lung tissue -
Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis
IF: 1.700Journal： Asian Pacific Journal of Tropical Biomedicine (2023)Reactivity：RatSample Type：heart -
Selenium and zinc alleviate quaternary metal mixture -induced neurotoxicity in rats by inhibiting oxidative damage and modulating the expressions of NF-kB and Nrf2/Hmox-1 pathway
IF: 1.500Journal： IBRO Neuroscience Reports (2023)PMID：37415728Reactivity：RatSample Type：cerebral cortex,cerebellum -
96th-hour impact of scalding burns on end organ damage, systemic oxidative stress, and wound healing in rats treated with three different types of dressings
IF: 1.400Journal： Journal of Burn Care & Research (2023)PMID：38079377Reactivity：RatSample Type：liver tissue,kidney tissue,lung tissue -
Glutamine prevents upregulation of NF-kB signaling and caspase 3 activation in ischaemia/reperfusion-induced testicular damage: An animal model
IF: 7.419Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)PMID：35658227Reactivity：RatSample Type：testicular tissue -
Antioxidant and anti-apoptotic effects of tocotrienol-rich fraction against streptozotocin-induced diabetic retinopathy in rats
IF: 7.419
实验操作视频
