Rat CASP3(Caspase 3) ELISA Kit
中文名称：大鼠胱天蛋白酶3(CASP3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CASP3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CASP3会与包被抗体结合。后依次加入生物素化的抗大鼠CASP3抗体和辣根过氧化物酶标记的亲和素，抗大鼠CASP3抗体与结合在包被抗体上的大鼠CASP3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CASP3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CASP3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CASP3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Co-exposure to aflatoxin B1 and therapeutic coartem worsens hepatic and renal function through enhanced oxido-inflammatory responses and apoptosis in rats
IF: 3.035 -
Upregulation of Uric Acid Production and Caspase 3 Signalling Mediates Rohypnol-Induced Cardiorenal Damage
IF: 2.755 -
The protective effect of 3-indolepropanoic acid on aflatoxin B1-induced systemic perturbation of the liver and kidney function in rats
IF: 2.747Journal： FUNDAMENTAL & CLINICAL PHARMACOLOGY (2022)PMID：36214208Reactivity：RatSample Type：liver, kidney -
Accidental lead in contaminated pipe-borne water and dietary furan intake perturbs rats’ hepatorenal function altering oxidative, inflammatory, and apoptotic balance
IF: 2.605Journal： BMC Pharmacology & Toxicology (2022)PMID：36180958Reactivity：RatSample Type：hepatic, renal -
Modulation of mitochondrial permeability transition pore opening by Myricetin and prediction of its-drug-like potential using in silico approach
IF: 2.597Journal： DRUG AND CHEMICAL TOXICOLOGY (2022)Reactivity：RatSample Type：liver mitochondria -
Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine
IF: 2.363Journal： Metabolic Syndrome and Related Disorders (2022)PMID：35796694Reactivity：RatSample Type：Liver tissue -
Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats
Journal： Current Research in Toxicology (2022)PMID：36624872Reactivity：RatSample Type：ovarian,thyroid -
Escitalopram Targets Oxidative Stress, Caspase-3, BDNF and MeCP2 in the Hippocampus and Frontal Cortex of a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress
IF: 5.924Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID：34299103Reactivity：RatSample Type：Hippocampus -
Therapeutic Potentials of Selected Antihypertensive Agents and Their Fixed-Dose Combinations Against Trastuzumab-Mediated Cardiotoxicity
IF: 5.811 -
Plumbagin induces testicular damage via mitochondrial-dependent cell death
IF: 5.194
实验操作视频
