Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
中文名称：大鼠肌酸激酶同工酶MB(CKMB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CKMB抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CKMB会与包被抗体结合。后依次加入生物素化的抗大鼠CKMB抗体和辣根过氧化物酶标记的亲和素，抗大鼠CKMB抗体与结合在包被抗体上的大鼠CKMB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CKMB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CKMB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CKMB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Catecholamines-based myocardial injury after acute ischemic stroke and effects of Naoxintong capsule therapy
IF: 6.700 -
Mitigating Doxorubicin-Induced Cardiotoxicity through Quercetin Intervention: An Experimental Study in Rats
IF: 6.000 -
Ultrasound?targeted microbubble destruction technology delivering β?klotho to the heart enhances FGF21 sensitivity and attenuates heart remodeling post?myocardial infarction
IF: 5.700Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2024)Reactivity：RatSample Type：serum -
Canagliflozin attenuates post-resuscitation myocardial dysfunction in diabetic rats by inhibiting autophagy through the PI3K/Akt/mTOR pathway
IF: 4.600Journal： iScience (2024)Reactivity：RatSample Type：Myocardial tissue -
Potential cardiac-derived exosomal miRNAs involved in cardiac healing and remodeling after myocardial ischemia–reperfusion injury
IF: 3.800 -
miR-652-3p Suppressed the Protective Effects of Isoflurane Against Myocardial Injury in Hypoxia/Reoxygenation by Targeting ISL1
IF: 3.400 -
Sirt3 Mediates the Cardioprotective Effect of Therapeutic Hypothermia after Cardiac Arrest and Resuscitation by Restoring Autophagic Flux via the PI3K/Akt/mTOR Pathway
IF: 3.100 -
Protective effects of arbutin against doxorubicin-induced cardiac damage
IF: 2.600Journal： MOLECULAR BIOLOGY REPORTS (2024)Reactivity：RatSample Type：serum -
Farrerol Alleviates Diabetic Cardiomyopathy by Regulating AMPK-Mediated Cardiac Lipid Metabolic Pathways in Type 2 Diabetic Rats
IF: 1.800Journal： CELL BIOCHEMISTRY AND BIOPHYSICS (2024)Reactivity：RatSample Type：serum -
Extracellular vesicles encapsulated with caspase-1 inhibitor ameliorate experimental autoimmune myasthenia gravis through targeting macrophages
IF: 10.800
实验操作视频
