Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
中文名称：大鼠肌酸激酶同工酶MB(CKMB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CKMB抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CKMB会与包被抗体结合。后依次加入生物素化的抗大鼠CKMB抗体和辣根过氧化物酶标记的亲和素，抗大鼠CKMB抗体与结合在包被抗体上的大鼠CKMB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CKMB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CKMB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CKMB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Vagus Nerve Stimulation Attenuates Septic Shock-Induced Cardiac Injury in Rats
IF: 2.100Journal： PHYSIOLOGICAL RESEARCH (2023)Reactivity：RatSample Type：serum -
Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia?reperfusion injury
IF: 9.429 -
Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis
IF: 7.419 -
Cardioprotective Effects of Aconite in Isoproterenol-Induced Myocardial Infarction in Rats
IF: 7.310Journal： Oxidative Medicine and Cellular Longevity (2022)PMID：36600948Reactivity：RatSample Type：serum -
Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway
IF: 6.780 -
Trimetazidine Alleviates Postresuscitation Myocardial Dysfunction and Improves 96‐Hour Survival in a Ventricular Fibrillation Rat Model
IF: 6.106Journal： Journal of the American Heart Association (2022)PMID：35261264Reactivity：RatSample Type：plasma -
Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury via the cAMP/PKA Signaling Pathway
IF: 5.846 -
A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation
IF: 5.195 -
Evidence-based mechanistic role of chrysin towards protection of cardiac hypertrophy and fibrosis in rats
IF: 4.125 -
Role of Keap1-Nrf2/ARE signal transduction pathway in protection of dexmedetomidine preconditioning against myocardial ischemia/reperfusion injury
IF: 3.976
实验操作视频
