Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
中文名称：大鼠肌酸激酶同工酶MB(CKMB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CKMB抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CKMB会与包被抗体结合。后依次加入生物素化的抗大鼠CKMB抗体和辣根过氧化物酶标记的亲和素，抗大鼠CKMB抗体与结合在包被抗体上的大鼠CKMB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CKMB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CKMB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CKMB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Activation of AMPK/p38/Nrf2 is involved in resveratrol alleviating myocardial ischemia-reperfusion injury in diabetic rats as an endogenous antioxidant stress feedback
IF: 3.616 -
Cissus quadrangularis extract mitigates diabetic cardiomyopathy by inhibiting RAAS activation, inflammation and oxidative stress
IF: 2.663 -
Carnosic Acid Protects Against Myocardial Infarction by Controlling Oxidative Stress and Inflammation in Rats
IF: 2.464Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2022)Reactivity：RatSample Type：serum -
The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury
IF: 14.919 -
microRNA-454-mediated NEDD4-2/TrkA/cAMP axis in heart failure: Mechanisms and cardioprotective implications
IF: 5.310Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)PMID：33949117Reactivity：RatSample Type：Plasma -
Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress
IF: 4.432 -
Antioxidant, anti-inflammatory, and anti-apoptotic effects of crocin against doxorubicin-induced myocardial toxicity in rats
IF: 4.223Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021)PMID：34322808Reactivity：RatSample Type：serum -
Effects of pinealectomy and crocin treatment on rats with isoproterenol-induced myocardial infarction
IF: 3.356Journal： DRUG AND CHEMICAL TOXICOLOGY (2021)Reactivity：RatSample Type：serum -
Dynamic observation of 5?fluorouracil?induced myocardial injury and mitochondrial autophagy in aging rats
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34721693Reactivity：RatSample Type：serum -
The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572927Reactivity：RatSample Type：Serum
实验操作视频
