Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
中文名称：大鼠肌酸激酶同工酶MB(CKMB)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CKMB抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CKMB会与包被抗体结合。后依次加入生物素化的抗大鼠CKMB抗体和辣根过氧化物酶标记的亲和素，抗大鼠CKMB抗体与结合在包被抗体上的大鼠CKMB结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CKMB浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CKMB的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CKMB，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism
IF: 2.971 -
Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase?1/IL?1β Signaling Pathway Inhibition
IF: 2.511Journal： IMMUNOLOGICAL INVESTIGATIONS (2020)PMID：32674625Reactivity：RatSample Type：Cell culture supernatant -
Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation
IF: 3.049 -
Hesperidin regresses cardiac hypertrophy by virtue of PPAR-γ agonistic, anti-inflammatory, antiapoptotic, and antioxidant properties
IF: 2.965Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019)PMID：30623541Reactivity：RatSample Type：Serum -
Bisoprolol and linagliptin ameliorated electrical and mechanical isometric myocardial contractions in doxorubicin-induced cardiomyopathy in rats
IF: 2.761 -
Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction
IF: 1.654Journal： KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY (2019)PMID：31080351Reactivity：RatSample Type：serum -
Resveratrol increase myocardial Nrf2 expression in type 2 diabetic rats and alleviate myocardial ischemia/reperfusion injury (MIRI)
IF: 1.262Journal： Annals of Palliative Medicine (2019)Reactivity：RatSample Type：blood -
Tongmai Yangxin pills anti-oxidative stress alleviates cisplatin-induced cardiotoxicity: Network pharmacology analysis and experimental evidence
IF: 3.457 -
Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia-reperfusion injury and its mechanism of UTR-RhoA-ROCK pathway inhibition
IF: 2.309Journal： JOURNAL OF PHARMACY AND PHARMACOLOGY (2018)PMID：30251312Reactivity：RatSample Type：Tissue homogenate -
AMPK activation restores ischemic post?conditioning cardioprotection in STZ?induced type 1 diabetic rats: Role of autophagy
IF: 1.692
实验操作视频
