Rat COL1(Collagen Type Ⅰ) ELISA Kit
中文名称：大鼠Ⅰ型胶原(COL1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 7.81-500 ng/mL
- 灵敏度： 4.69 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠COL1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠COL1会与包被抗体结合。后依次加入生物素化的抗大鼠COL1抗体和辣根过氧化物酶标记的亲和素，抗大鼠COL1抗体与结合在包被抗体上的大鼠COL1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，COL1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中COL1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 ng/mL |
| 灵敏度 | 4.69 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠COL1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 5.100 -
Design, Synthesis, and Biological Evaluation of Novel Evodiamine Derivatives as Potential Antihepatocellular Carcinoma Agents
IF: 8.039 -
Collagen Deposition and Inflammatory Response Associated with Macroporous Mesh Shrinkage in Incisional Hernia Repair: A Rat Model
IF: 1.972Journal： JOURNAL OF INVESTIGATIVE SURGERY (2022)Reactivity：RatSample Type：anterior abdominal wall -
Effective-components combination improves airway remodeling in COPD rats by suppressing M2 macrophage polarization via the inhibition of mTORC2 activity
IF: 5.340Journal： PHYTOMEDICINE (2021)PMID：34600177Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
Wound healing potential of human umbilical cord mesenchymal stem cell conditioned medium: An in vitro and in vivo study in diabetes-induced rats
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Effects of Secreted frizzled-related protein 1 on inhibiting cardiac remodeling
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572894Reactivity：RatSample Type：Cell culture supernatant -
Effects of cannabinoid receptor 2 synthetic agonist, AM1241, on bleomycin induced pulmonary fibrosis
IF: 1.203Journal： BIOTECHNIC & HISTOCHEMISTRY (2020)PMID：33325762Reactivity：RatSample Type：Tissue homogenate -
Design, synthesis and biological evaluation of non-secosteriodal vitamin D receptor ligand bearing double side chain for the treatment of chronic pancreatitis
IF: 4.816Journal： EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2018)PMID：29407979Reactivity：RatSample Type：Cell culture supernatant -
Comparative evaluation of the biocompatible and physical–chemical properties of poly(lactide-co-glycolide) and polydopamine as coating materials for bacterial cellulose
IF: 4.776 -
Inhibiting autophagy promotes collagen degradation by regulating matrix metalloproteinases in pancreatic stellate cells
IF: 3.234
实验操作视频
