Rat COL3(Collagen Type Ⅲ) ELISA Kit
中文名称：大鼠Ⅲ型胶原(COL3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠COL3抗体包被于酶标板上，实验时样品（或标准品）中的大鼠COL3会与包被抗体结合。后依次加入生物素化的抗大鼠COL3抗体和辣根过氧化物酶标记的亲和素，抗大鼠COL3抗体与结合在包被抗体上的大鼠COL3结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，COL3浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中COL3的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠COL3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effect of Stanozolol and/or Cannabis Abuse on Hypertrophic Mechanism and Oxidative Stress of Male Albino Rat Cardiac Tissue in Relation to Exercise: A Sport Abuse Practice
IF: 3.400Journal： Cardiovascular Toxicology (2024)Reactivity：RatSample Type：Cardiac Tissue -
Direct Detection of Pulmonary Fibrosis by Near-Infrared-Responsive Biomimetic Platelets
IF: 7.033Journal： International Journal of Nanomedicine (2022)PMID：35046651Reactivity：RatSample Type：lung tissue -
The association between obesity and vitamin D deficiency modifies the progression of kidney disease after ischemia/reperfusion injury
IF: 6.590Journal： Frontiers in Nutrition (2022)Reactivity：RatSample Type：renal tissue -
Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.
IF: 5.058 -
Collagen Deposition and Inflammatory Response Associated with Macroporous Mesh Shrinkage in Incisional Hernia Repair: A Rat Model
IF: 1.972Journal： JOURNAL OF INVESTIGATIVE SURGERY (2022)Reactivity：RatSample Type：anterior abdominal wall -
Effective-components combination improves airway remodeling in COPD rats by suppressing M2 macrophage polarization via the inhibition of mTORC2 activity
IF: 5.340Journal： PHYTOMEDICINE (2021)PMID：34600177Reactivity：RatSample Type：bronchoalveolar lavage fluid (BALF) -
Effects of Secreted frizzled-related protein 1 on inhibiting cardiac remodeling
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572894Reactivity：RatSample Type：Cell culture supernatant -
Inhibiting autophagy promotes collagen degradation by regulating matrix metalloproteinases in pancreatic stellate cells
IF: 3.234 -
Anti-Adhesion Mesh for Hernia Repair Based on Modified Bacterial Cellulose
IF: 2.173 -
Study of synergistic role of allogenic skin-derived precursor differentiated Schwann cells and heregulin-1β in nerve regeneration with an acellular nerve allograft
IF: 3.385Journal： NEUROCHEMISTRY INTERNATIONAL (2016)PMID：27063890Reactivity：RatSample Type：Tissue homogenate
实验操作视频
