Rat CRP(C-Reactive Protein) ELISA Kit
中文名称：大鼠C反应蛋白(CRP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CRP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CRP会与包被抗体结合。后依次加入生物素化的抗大鼠CRP抗体和辣根过氧化物酶标记的亲和素，抗大鼠CRP抗体与结合在包被抗体上的大鼠CRP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CRP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CRP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CRP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Investigating the association between diet-induced “leaky gut” and the development of prediabetes
IF: 1.800Journal： EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2023)PMID：37751850Reactivity：RatSample Type：plasma -
Antioxidant, anti-inflammatory, and anti-reprotoxic effects of kaempferol and vitamin E on lead acetate-induced testicular toxicity in male rats
IF: 1.200Journal： Open Veterinary Journal (2023)Reactivity：RatSample Type：Testicular -
The dual gastro- and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats
IF: 7.419 -
Body, metabolic and renal changes following cross-sex estrogen/progestogen therapy in a rodent model simulating its use by transwomen
IF: 5.467Journal： JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION (2022)PMID：35689728Reactivity：RatSample Type：serum -
Validation of traditional claims of anti-arthritic efficacy of trans-Himalayan snow mountain garlic (Allium ampeloprasum L.) extract using adjuvant-induced arthritis rat model: A comparative evaluation with normal garlic (Allium sativum L.) and dexamethasone
IF: 5.195 -
Network pharmacology combined with pharmacodynamics revealed the anti-inflammatory mechanism of Tanreqing capsule against acute-exacerbation chronic obstructive pulmonary disease
IF: 4.996 -
Kinetin Ameliorates Cisplatin-Induced Hepatotoxicity and Lymphotoxicity via Attenuating Oxidative Damage, Cell Apoptosis and Inflammation in Rats
IF: 4.757 -
Gastroprotective Effect of Azilsartan Through Ameliorating Oxidative Stress, Inflammation, and Restoring Hydroxyproline, and Gastrin Levels in Ethanol-Induced Gastric Ulcer
IF: 4.631 -
Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid
IF: 4.614Journal： International Journal of Environmental Research and Public Health (2022)PMID：35805580Reactivity：RatSample Type：plasma -
The effect of TNF-α inhibitor treatment on microRNAs and endothelial function in collagen induced arthritis
IF: 3.752
实验操作视频
