Rat CRP(C-Reactive Protein) ELISA Kit
中文名称：大鼠C反应蛋白(CRP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CRP抗体包被于酶标板上，实验时样品（或标准品）中的大鼠CRP会与包被抗体结合。后依次加入生物素化的抗大鼠CRP抗体和辣根过氧化物酶标记的亲和素，抗大鼠CRP抗体与结合在包被抗体上的大鼠CRP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，CRP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中CRP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠CRP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Tert-butylhydroquinone abrogates fructose-induced insulin resistance in rats via mitigation of oxidant stress, NFkB-mediated inflammation in the liver but not the skeletal muscle of high fructose drinking rats
IF: 3.654 -
Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats
IF: 3.249Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022)PMID：30858714Reactivity：RatSample Type：Tissue homogenate,Plasma -
Justicia carnea extracts ameliorated hepatocellular damage in?streptozotocin-induced type 1 diabetic male rats via decrease in?oxidative stress, inflammation and increasing other risk markers
IF: 2.663 -
Chronic oral trimethylamine-N-oxide administration induces experimental incipient atherosclerosis in non-genetically modified mice
IF: 2.589Journal： JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2022)Reactivity：RatSample Type：blood -
Increased protein phosphatase 5 expression in inflammation-induced left ventricular dysfunction in rats
IF: 2.174 -
Comparison of the healing effects of mesazaline and Ganoderma lucidum in acetic acid-induced colitis in rats
IF: 1.766Journal： Annals of Surgical Treatment and Research (2022)PMID：35071117Reactivity：RatSample Type：colonic tissue -
Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats
-
Anti-Inflammatory Potentials of the n-Hexane Fraction of Alstonia boonei Stem Bark in Lipopolysaccharide-Induced Inflammation in Wistar Rats
IF: 6.922 -
Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation
IF: 6.530 -
Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model
IF: 5.820
实验操作视频
