Rat CRP(C-Reactive Protein) ELISA Kit
中文名称:大鼠C反应蛋白(CRP)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.1 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠CRP抗体包被于酶标板上,实验时样品(或标准品)中的大鼠CRP会与包被抗体结合。后依次加入生物素化的抗大鼠CRP抗体和辣根过氧化物酶标记的亲和素,抗大鼠CRP抗体与结合在包被抗体上的大鼠CRP结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,CRP浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中CRP的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.1 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠CRP,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Increased protein phosphatase 5 expression in inflammation-induced left ventricular dysfunction in rats
IF: 2.174 -
Comparison of the healing effects of mesazaline and Ganoderma lucidum in acetic acid-induced colitis in rats
IF: 1.766Journal: Annals of Surgical Treatment and Research (2022)PMID:35071117Reactivity:RatSample Type:colonic tissue -
Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats
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Anti-Inflammatory Potentials of the n-Hexane Fraction of Alstonia boonei Stem Bark in Lipopolysaccharide-Induced Inflammation in Wistar Rats
IF: 6.922 -
Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation
IF: 6.530 -
Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model
IF: 5.820 -
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats
IF: 4.432Journal: EUROPEAN JOURNAL OF PHARMACOLOGY (2021)PMID:33745959Reactivity:RatSample Type:Tissue homogenate(Joint Tissue) -
Quality of Dabai Pulp Oil Extracted by Supercritical Carbon Dioxide and Supplementation in Hypercholesterolemic Rat—A New Alternative Fat
IF: 4.350 -
Diet-induced prediabetes: effects of exercise treatment on risk factors for cardiovascular complications
IF: 4.169Journal: Nutrition & Metabolism (2021)PMID:33888141Reactivity:RatSample Type:Tissue homogenate(heart) -
Neuropeptide W Attenuates Oxidative Multi-Organ Injury in Rats Induced with Intra-Abdominal Sepsis
IF: 4.092
