Rat Cys-C(Cystatin C) ELISA Kit
中文名称：大鼠胱抑素C(Cys-C)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠Cys-C抗体包被于酶标板上，实验时样品（或标准品）中的大鼠Cys-C会与包被抗体结合。后依次加入生物素化的抗大鼠Cys-C抗体和辣根过氧化物酶标记的亲和素，抗大鼠Cys-C抗体与结合在包被抗体上的大鼠Cys-C结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Cys-C浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Cys-C的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Cys-C，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Comparative effects of incretin-based therapy on doxorubicin-induced nephrotoxicity in rats: the role of SIRT1/Nrf2/NF-κB/TNF-α signaling pathways
IF: 5.600 -
Cyclosporine-induced kidney damage was halted by sitagliptin and hesperidin via increasing Nrf2 and suppressing TNF-α, NF-κB, and Bax
IF: 4.600 -
Piperazine ferulate inhibits diabetic nephropathy by suppressing AGE/RAGE-mediated inflammatory signaling in rats and podocytes
IF: 4.400Journal： Frontiers in Pharmacology (2024)Reactivity：RatSample Type：serum -
Exploring the impact of high-altitude de-acclimatization on renal function: The roles of oxidative and endoplasmic reticulum stress in rat models
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)PMID：38518722Reactivity：RatSample Type：serum,urine,renal tissue -
Multiple-heated cooking oil promotes early hepatic and renal senescence in adult male rats: The potential regenerative capacity of oleuropein.
IF: 2.800Journal： TOXICOLOGY MECHANISMS AND METHODS (2024)Reactivity：RatSample Type：serum -
The Nephroprotective Effect of Cornelian Cherry (Cornus mas L.) and Rowanberry (Sorbus aucuparia L.) in Gentamicin-Induced Nephrotoxicity on Wistar Rats with Emphasis on the Evaluation of Novel Renal Biomarkers and the Antioxidant Capacity in Correlation with Nitro-Oxidative Stress
IF: 5.900 -
Shikonin Alleviates Gentamicin-Induced Renal Injury in Rats by Targeting Renal Endocytosis, SIRT1/Nrf2/HO-1, TLR-4/NF-κB/MAPK, and PI3K/Akt Cascades
IF: 4.800 -
Caution may be required in using l-theanine in diabetes mellitus: A study on the rats
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37199135Reactivity：RatSample Type：Kidney,heart -
Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats
IF: 7.109 -
Angiotensin 1–7 mitigates rhabdomyolysis induced renal injury in rats via modulation of TLR-4/NF-kB/iNOS and Nrf-2/heme?oxygenase-1 signaling pathways
IF: 6.780
实验操作视频
