Rat EPO(Erythropoietin) ELISA Kit
中文名称：大鼠促红细胞生成素(EPO)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠EPO抗体包被于酶标板上，实验时样品（或标准品）中的大鼠EPO会与包被抗体结合。后依次加入生物素化的抗大鼠EPO抗体和辣根过氧化物酶标记的亲和素，抗大鼠EPO抗体与结合在包被抗体上的大鼠EPO结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，EPO浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中EPO的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠EPO，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Multiscale effects of the calcimimetic drug, etelcalcetide on bone health of rats with secondary hyperparathyroidism induced by chronic kidney disease
IF: 3.500 -
Physical exercise improved the hematological effect of vitamin D in type 2 diabetes mellitus-induced nephrotoxicity in rats
IF: 2.300Journal： Biochemistry and Biophysics Reports (2024)Reactivity：RatSample Type：Plasma -
Protective effects of epigallocatechin-3-gallate counteracting the chronic hypobaric hypoxia-induced myocardial injury in plain-grown rats at high altitude
IF: 3.800 -
Anemia Recovery After Lung Contusion, Hemorrhagic Shock, and Chronic Stress Is Gender-Specific in a Rat Model
IF: 2.000 -
Ameliorative Effects of Bovine Lactoferrin on Benzene-Induced Hematotoxicity in Albino Rats
IF: 3.471 -
Selective HIF stabilization alleviates hepatocellular steatosis and ballooning in a rodent model of 70% liver resection
IF: 6.124 -
Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats
IF: 3.168Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021)PMID：34707382Reactivity：RatSample Type：plasma -
In anemia zinc is recruited from bone and plasma to produce new red blood cells
IF: 3.212 -
Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin
IF: 4.183Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)PMID：31600973Reactivity：RatSample Type：Cell culture supernatant -
The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers
IF: 3.743
实验操作视频
