Rat ET-1(Endothelin 1) ELISA Kit
中文名称：大鼠内皮素1(ET-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.78-50 pg/mL
- 灵敏度： 0.47 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ET-1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠ET-1会与包被抗体结合。后依次加入生物素化的抗大鼠ET-1抗体和辣根过氧化物酶标记的亲和素，抗大鼠ET-1抗体与结合在包被抗体上的大鼠ET-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ET-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ET-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 pg/mL |
| 灵敏度 | 0.47 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ET-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Laxative effect and mechanism of Tiantian Capsule on loperamide-induced constipation in rats
IF: 3.690 -
Emerging roles of let?7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation
IF: 3.098Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2020)PMID：32319531Reactivity：RatSample Type：lung -
Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism
IF: 2.971 -
Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats
IF: 2.575Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020)PMID：32584611Reactivity：RatSample Type：Serum -
Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist
IF: 1.862 -
Chronic intermittent hypoxia vs chronic continuous hypoxia: Effects on vascular endothelial function and myocardial contractility
IF: 1.741Journal： CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020)PMID：31683472Reactivity：RatSample Type：serum -
Regulation of PI3k-WNK Pathway by Ethyl Acetate Partition Fraction of Gardenia jasminoides var. radicans Makino in SHR, NRK52e Cells, and IMCD3 Cells
IF: 0.468Journal： Natural Product Communications (2020)Reactivity：RatSample Type：Serum -
Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells
IF: 2.197Journal： Biomed Research International (2019)PMID：30993112Reactivity：RatSample Type：Cell culture supernatant -
Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system
IF: 2.107 -
Piper sarmentosum Leaves Aqueous Extract Attenuates Vascular Endothelial Dysfunction in Spontaneously Hypertensive Rats
IF: 1.984Journal： Evidence-based Complementary and Alternative Medicine (2019)PMID：31485247Reactivity：RatSample Type：mesenteric artery
实验操作视频
