Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 ng/mL
- 灵敏度: 1.88 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 3.13-200 ng/mL |
灵敏度 | 1.88 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone
IF: 3.251 -
Targeting p-AKT/mTOR/MAP kinase signaling, NLRP3 inflammasome and apoptosis by fluvastatin with or without taxifolin mitigates gonadal dysfunction induced by bisphenol-A in male rats.
IF: 3.247 -
Effects of intracerebroventricular and intravenous administration of Kisspeptin-54 and Gonadotropin-releasing hormone agonist in rats with ovarian hyperstimulation
IF: 3.152 -
Male reproductive system and simulated high-altitude environment: preliminary results in rats
IF: 3.054 -
Harmful Consequences of Proton Pump Inhibitors on Male Fertility: An Evidence from Subchronic Toxicity Study of Esomeprazole and Lansoprazole in Wistar Rats
IF: 2.803Journal: International Journal of Endocrinology (2022)PMID:35529080Reactivity:RatSample Type:serum -
Capsaicin prevents radiotherapy-induced premature ovarian failure in rats
IF: 1.973Journal: REPRODUCTION FERTILITY AND DEVELOPMENT (2022)DOI:10.1071/RD21235PMID:35101163Reactivity:RatSample Type:serum -
Regulation of male sex hormones strongly heightens copulation behavior indices in rat’s model administered Cocos nucifera husks extract
IF: 1.108 -
Potential use of Indonesian basil (Ocimum basilicum) maceration to increase estradiol and progesterone synthesis and secretion to improve prenatal growth of offspring using female albino rats as an animal model
-
The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway
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Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats
IF: 7.963