Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称：大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素，抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FSH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FSH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠FSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of prenatal chlorocholine chloride exposure on pubertal development and reproduction of male offspring in rats
IF: 4.374 -
Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet
IF: 4.310Journal： AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021)PMID：34229480Reactivity：RatSample Type：serum, testis -
Benzo-a-pyrene-induced reproductive toxicity was abated in rats co-treated with taurine
IF: 4.266 -
Intracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in rats
IF: 4.102 -
CXCL12 and its receptors regulate granulosa cell apoptosis in PCOS rats and human KGN tumor cells
IF: 3.906Journal： REPRODUCTION (2021)PMID：33258800Reactivity：RatSample Type：Serum,Cell culture supernatant(ovarian granulosa cells) -
Aluminum Chloride–Induced Reproductive Toxicity in Rats: the Protective Role of Zinc Oxide Nanoparticles
IF: 3.738 -
Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats
IF: 3.652Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021)PMID：33592137Reactivity：RatSample Type：Serum -
Repression of HDAC5 by acetate restores hypothalamic-pituitary-ovarian function in type 2 diabetes mellitus
IF: 3.143 -
Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses
IF: 3.060 -
Human Amnion Membrane-Derived Mesenchymal Stem Cells and Conditioned Medium Can Ameliorate X-Irradiation-Induced Testicular Injury by Reducing Endoplasmic Reticulum Stress and Apoptosis
IF: 3.060
实验操作视频
