Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 ng/mL
- 灵敏度: 1.88 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 3.13-200 ng/mL |
灵敏度 | 1.88 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Effects of intracerebroventricular administration of irisin on the hypothalamus–pituitary–gonadal axis in male rats
IF: 3.923 -
Sleeve Gastrectomy Reversed Obesity-Induced Hypogonadism in a Rat Model by Regulating Inflammatory Responses in the Hypothalamus and Testis
IF: 3.890 -
Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats
IF: 3.125 -
GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile
IF: 2.852Journal: Reproductive Biology and Endocrinology (2018)PMID:29642911Reactivity:RatSample Type:Serum -
Mitochondria-targeted antioxidant therapy for an animal model of PCOS-IR
IF: 2.784Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID:30431108Reactivity:RatSample Type:Serum -
Expression of FKBP52 in the ovaries of PCOS rats
IF: 2.784Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018)PMID:30483787Reactivity:RatSample Type:Serum -
Virgin coconut oil extract mitigates testicular-induced toxicity of alcohol use in antiretroviral therapy
IF: 2.734 -
Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging
IF: 2.517 -
Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment
IF: 2.064Journal: Evidence-based Complementary and Alternative Medicine (2018)PMID:29770151Reactivity:RatSample Type:serum -
Melatonin prevents and ameliorates lead-induced gonadotoxicity through antioxidative and hormonal mechanisms
IF: 1.255

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