Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称：大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素，抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，FSH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中FSH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠FSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Mechanism of ferroptosis in a rat model of premature ovarian insufficiency induced by cisplatin
IF: 4.600 -
Effect of Vitamin D on the Proliferation and Barrier of Atrophic Vaginal Epithelial Cells
IF: 4.600 -
Exogenous taurine administration abates reproductive dysfunction in male rats exposed to silver nanoparticles
IF: 4.500 -
Exercise-induced irisin improves follicular dysfunction by inhibiting IRE1α-TXNIP/ROS-NLRP3 pathway in PCOS
IF: 4.000 -
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model
IF: 4.000 -
Bushen Huoxue formula for the treatment of diminished ovarian reserve: A combined metabolomics and integrated network pharmacology analysis
IF: 4.000 -
Prenatal cadmium exposure has intergenerational adverse effects on the Sertoli cells through FSHR pathway
IF: 3.800 -
Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37129605Reactivity：RatSample Type：serum -
Naringenin alleviates the excessive lipid deposition of polycystic ovary syndrome rats and insulin-resistant adipocytes by promoting PKGIα
IF: 3.600Journal： AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023)PMID：38009056Reactivity：RatSample Type：serum -
Attenuation of doxorubicin-induced hypothalamic-pituitary-testicular axis dysfunction by diphenyl diselenide involves suppression of hormonal deficits, oxido-inflammatory stress and caspase 3 activity in rats
IF: 3.500Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2023)PMID：37379681Reactivity：RatSample Type：serum
实验操作视频
