Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 ng/mL
- 灵敏度: 1.88 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 3.13-200 ng/mL |
灵敏度 | 1.88 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145–5p
IF: 3.400 -
Investigation of irisin's role in pubertal onset physiology in female rats
IF: 3.000 -
Effect of methylphenidate on the onset of puberty and reproductive organ development in rats
IF: 2.900 -
Thymol co-administration abrogates hexachlorobenzene-induced reproductive toxicities in male rats
IF: 2.800 -
Chemoprotective Effect of Plantago sempervirens Crantz Extract on Ovarian Structure and Folliculogenesis
IF: 2.700 -
Impact of Concurrent Exposure of Diabetic Male Sprague Dawley Rats to Alcohol and Combination Antiretroviral Therapy (cART) on Reproductive Capacity
IF: 2.700 -
Hydrogen-rich water treatment targets RT1-Db1 and RT1-Bb to alleviate premature ovarian failure in rats
IF: 2.700 -
Melatonin effective to reduce the microscopic symptoms of polycystic ovary syndrome-related infertility: An experimental study
IF: 2.600 -
HGF-modified human umbilical cord mesenchymal stem cells rescue impaired ovarian reserve function in chemotherapy-induced POI rats by improving angiogenesis while decreasing apoptosis and fibrosis in the ovary
IF: 2.600 -
Co-administration of thymol and sulfoxaflor impedes the expression of reproductive toxicity in male rats
IF: 2.600