Rat FSH(Follicle Stimulating Hormone) ELISA Kit
中文名称:大鼠促卵泡激素(FSH)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 ng/mL
- 灵敏度: 1.88 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠FSH抗体包被于酶标板上,实验时样品(或标准品)中的大鼠FSH会与包被抗体结合。后依次加入生物素化的抗大鼠FSH抗体和辣根过氧化物酶标记的亲和素,抗大鼠FSH抗体与结合在包被抗体上的大鼠FSH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,FSH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中FSH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 3.13-200 ng/mL |
灵敏度 | 1.88 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠FSH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
操作步骤 | ![]() |
数据处理 |
-
Morphology and Cyto-Architecture of the Testis and Assessment of Selected Biochemical Parameters Associated with Male Fertility Following the Consumption of Tom Brown Weaning Diet in Albino Wistar Rats
IF: 1.8Journal: Natural Product Communications (2024)Reactivity:RatSample Type:serum -
Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats
IF: 1.6Journal: BIOTECHNIC & HISTOCHEMISTRY (2024)Reactivity:RatSample Type:serum -
Treatment of D-galactose-induced rat polycystic ovarian condition using Lepidium sativum and secondary antibodies
IF: 1.5Journal: Journal of Advanced Veterinary and Animal Research (2024)Reactivity:RatSample Type:serum -
Effect of maceration treatment on ovarian follicular development, serum oestradiol, uterine growth and vascularisation in female albino rats
IF: 1.3Journal: Journal of Veterinary Research (2024)Reactivity:RatSample Type:serum -
Cold exposure induces ovarian dysfunction through endoplasmic reticulum stress-mediated apoptosis
IF: 0.8 -
Phloretin Ameliorates against Bisphenol A Induced Polycystic Ovarian Syndrome, an Endocrine Disrupting Chemical, in Prepubertal Rats
IF: 0.5 -
Development of MDCK-based quadrivalent split seasonal influenza virus vaccine with high safety and immunoprotection: A preclinical study
IF: 7.6 -
17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD)
IF: 7.0 -
Copper nanoparticles lead to reproductive dysfunction by affecting key enzymes of ovarian hormone synthesis and metabolism in female rats
IF: 6.800 -
Acupuncture regulates the apoptosis of ovarian granulosa cells in polycystic ovarian syndrome-related abnormal follicular development through LncMEG3-mediated inhibition of miR-21-3p
IF: 6.7

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