Rat GC(Glucagon) ELISA Kit
中文名称：大鼠胰高血糖素(GC)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Rat
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠GC抗原包被于酶标板上，实验时样品或标准品中的大鼠GC与包被的大鼠GC竞争生物素标记的抗大鼠GC单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠GC浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠GC的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GC，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Comparative efficacy of four potent bioactive molecules of fenugreek seed in holistic management of type 1 diabetes
IF: 1.800Journal： Advances in Traditional Medicine (2024)Reactivity：RatSample Type：serumMetabolic and Molecular Response to High-Fat Diet Differs between Rats with Constitutionally High and Low Serotonin Tone
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：36768493Reactivity：RatSample Type：plasmaPancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production
IF: 5.000PDHA1 Alleviates Myocardial Ischemia-Reperfusion Injury by Improving Myocardial Insulin Resistance During Cardiopulmonary Bypass Surgery in Rats
IF: 3.400Journal： CARDIOVASCULAR DRUGS AND THERAPY (2023)PMID：37610688Reactivity：RatSample Type：Serum,myocardial tissueβ-Sitosterol Glucoside-Loaded Nanosystem Ameliorates Insulin Resistance and Oxidative Stress in Streptozotocin-Induced Diabetic Rats
IF: 7.675Kynurenine-3-monooxygenase expression is activated in the pancreatic endocrine cells by diabetes and its blockade improves glucose-stimulated insulin secretion
IF: 6.633Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2022)PMID：35914653Reactivity：RatSample Type：isletIntraductal botulinum toxin injection suppressed the inflammation in experimental acute pancreatitis.
IF: 0.894Journal： Ulusal Travma ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery (2022)PMID：36453789Reactivity：RatSample Type：bloodMetabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis
IF: 5.924Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID：34065591Reactivity：RatSample Type：PlasmaExcitatory Impact of Dental Occlusion on Dorsal Motor Nucleus of Vagus
IF: 3.492Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
IF: 6.018Journal： Computational and Structural Biotechnology Journal (2020)PMID：33304464Reactivity：RatSample Type：plasma
