Rat GH(Growth Hormone) ELISA Kit
中文名称：大鼠生长激素(GH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠GH抗体包被于酶标板上，实验时样品（或标准品）中的大鼠GH会与包被抗体结合。后依次加入生物素化的抗大鼠GH抗体和辣根过氧化物酶标记的亲和素，抗大鼠GH抗体与结合在包被抗体上的大鼠GH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Bovine lactopontin promotes bone development in growing rats by altering the composition of intestinal flora and bile acid metabolism
IF: 5.100 -
The disrupting effect of chlormequat chloride on growth hormone is associated with pregnancy
IF: 3.500 -
Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：37240089Reactivity：RatSample Type：serum -
Targeting the lncMST-EPRS/HSP90AB1 complex as novel therapeutic strategy for T-2 toxin-induced growth retardation
IF: 7.129Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)PMID：36332407Reactivity：RatSample Type：blood -
Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats
IF: 2.125 -
PPAR-γ with its anti-fibrotic action could serve as an effective therapeutic target in T-2 toxin-induced cardiac fibrosis of rats
IF: 6.025 -
Pathological changes and oxidative stress of the HPG axis in hypothyroid rat
IF: 5.098 -
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
IF: 6.018Journal： Computational and Structural Biotechnology Journal (2020)PMID：33304464Reactivity：HumanSample Type：blood -
Bee Venom Stimulates Hormone Secretion in Rat Somatotroph and Corticotroph Cells: Digital Image Analysis of Secretory Granules
IF: 2.673 -
AFAP1-AS1 Promotes Proliferation of Pituitary Adenoma Cells through miR-103a-3p to Activate PI3K/AKT Signaling Pathway
IF: 1.723
实验操作视频
