Rat GHRL(Ghrelin) ELISA Kit
中文名称：大鼠饥饿素(GHRL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠GHRL抗原包被于酶标板上，实验时样品或标准品中的大鼠GHRL与包被的大鼠GHRL竞争生物素标记的抗大鼠GHRL单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，大鼠GHRL浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中大鼠GHRL的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GHRL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Delphinidin or α-amyrin attenuated liver steatosis and metabolic disarrangement in rats fed a high-fat diet
IF: 5.000 -
The Influence of a High-Cholesterol Diet and Forced Training on Lipid Metabolism and Intestinal Microbiota in Male Wistar Rats
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：serum -
Effects of Gossypetin on Glucose Homeostasis in Diet-Induced Pre-Diabetic Rats
IF: 4.200 -
Establishing a Female Animal Model of Prediabetes Using a High-Carbohydrate, High-Fat Diet
IF: 2.800Journal： CURRENT ISSUES IN MOLECULAR BIOLOGY (2024)Reactivity：RatSample Type：plasma -
Momordica balsamina improves glucose handling in a diet-induced prediabetic rat model
IF: 3.700 -
The Effectiveness of?Nigella sativa?and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats
IF: 2.900 -
Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota
IF: 10.723 -
Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism
IF: 6.317 -
Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats
IF: 4.693 -
The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model
IF: 1.653
实验操作视频
