Rat GLP-1(Glucagon Like Peptide 1) ELISA Kit
中文名称：大鼠胰高血糖素样肽1(GLP-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠GLP-1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠GLP-1会与包被抗体结合。后依次加入生物素化的抗大鼠GLP-1抗体和辣根过氧化物酶标记的亲和素，抗大鼠GLP-1抗体与结合在包被抗体上的大鼠GLP-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GLP-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GLP-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GLP-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Omarigliptin/rosinidin combination ameliorates cyclophosphamide-induced lung toxicity in rats: The interaction between glucagon-like peptide-1, TXNIP/NLRP3 inflammasome signaling, and PI3K/Akt/FoxO1 axis
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：RatSample Type：lung tissue -
Zearalenone Decreases Food Intake by Disrupting the Gut–Liver–Hypothalamus Axis Signaling via Bile Acids
IF: 5.700Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2024)Reactivity：RatSample Type：serum -
Ramulus Mori (Sangzhi) alkaloids ameliorate high-fat diet induced obesity in rats by modulating gut microbiota and bile acid metabolism
IF: 3.900Journal： Frontiers in Endocrinology (2024)Reactivity：RatSample Type：serum -
Vitamin D3 mitigates myopathy and metabolic dysfunction in rats with metabolic syndrome: the potential role of dipeptidyl peptidase-4
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Protective effect of Dulaglutide, a GLP1 agonist, on acetic acid-induced ulcerative colitis in rats: involvement of GLP-1, TFF-3, and TGF-β/PI3K/NF-κB signaling pathway
IF: 3.100Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)Reactivity：RatSample Type：colonic tissue -
Comparative efficacy of four potent bioactive molecules of fenugreek seed in holistic management of type 1 diabetes
IF: 1.800Journal： Advances in Traditional Medicine (2024)Reactivity：RatSample Type：serum -
Antidiabetic impact of novel sophorolipids produced via microbial conversion of Moringa oleifera and Lepidium sativum oil cakes utilizing locally isolated yeast strains in streptozotocin-induced diabetic wistar rats: role of glucagon-like peptide-1
IF: 0.700Journal： Egyptian Pharmaceutical Journal (2024)Reactivity：RatSample Type：serum -
Melatonin-mediated IGF-1/GLP-1 activation in experimental OCD rats: Evidence from CSF, blood plasma, brain and in-silico investigations
IF: 5.800Journal： BIOCHEMICAL PHARMACOLOGY (2023)PMID：37777162Reactivity：RatSample Type：cerebrospinal fluid (CSF), brain,plasma -
The Effectiveness of?Nigella sativa?and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats
IF: 2.900 -
Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway
IF: 5.195
实验操作视频
