Rat GPX1(Glutathione Peroxidase 1) ELISA Kit
中文名称：大鼠谷胱甘肽过氧化酶1(GPX1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠GPX1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠GPX1会与包被抗体结合。后依次加入生物素化的抗大鼠GPX1抗体和辣根过氧化物酶标记的亲和素，抗大鼠GPX1抗体与结合在包被抗体上的大鼠GPX1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GPX1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GPX1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GPX1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Antioxidative Propolis From Stingless Bees (Heterotrigona Itama) Preserves Endothelium-Dependent Aortic Relaxation of Diabetic Rats: The Role of Nitric Oxide and Cyclic Guanosine Monophosphate
IF: 1.321Journal： Brazilian Journal of Pharmaceutical Sciences (2021)Reactivity：RatSample Type：Aortic tissue -
The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals
IF: 3.998 -
l-theanine alleviates liver and kidney dysfunction in septic rats induced by cecal ligation and puncture
IF: 3.647Journal： LIFE SCIENCES (2020)PMID：32142764Reactivity：RatSample Type：Cell culture supernatant,Tissue homogenate -
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades
IF: 3.647 -
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways
IF: 3.292Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020)PMID：32339907Reactivity：RatSample Type：Serum -
Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods
IF: 2.639Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2020)PMID：32594358Reactivity：RatSample Type：Tissue homogenate -
Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model
IF: 2.055Journal： Canadian Journal of Gastroenterology and Hepatology (2020)PMID：32149046Reactivity：RatSample Type：Tissue homogenate -
Pyrroloquinoline Quinone Inhibits Oxidative Stress in Rats with Diabetic Nephropathy
IF: 1.918 -
Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats
IF: 1.754 -
Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus
Journal： Advances in Traditional Medicine (2020)Reactivity：RatSample Type：Tissue homogenate
实验操作视频
