Rat GPX1(Glutathione Peroxidase 1) ELISA Kit
中文名称：大鼠谷胱甘肽过氧化酶1(GPX1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠GPX1抗体包被于酶标板上，实验时样品（或标准品）中的大鼠GPX1会与包被抗体结合。后依次加入生物素化的抗大鼠GPX1抗体和辣根过氧化物酶标记的亲和素，抗大鼠GPX1抗体与结合在包被抗体上的大鼠GPX1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，GPX1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中GPX1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠GPX1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Neuroprotective effects of Hericium erinaceus (Bull.: Fr.) Pers. against high-dose corticosterone-induced oxidative stress in PC-12 cells
Journal： BMC Complementary Medicine and Therapies (2020)PMID：33176761Reactivity：RatSample Type：Cell culture supernatant -
The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers
IF: 3.743Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)PMID：31112874Reactivity：RatSample Type：plasma,kidney tissues -
Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1
IF: 3.643Journal： SAUDI PHARMACEUTICAL JOURNAL (2019)PMID：31920438Reactivity：RatSample Type：Tissue homogenate -
The Haematological Effects of Oleanolic Acid in Streptozotocin-Induced Diabetic Rats: Effects on Selected Markers
IF: 3.040Journal： Journal of Diabetes Research (2019)PMID：31828165Reactivity：RatSample Type：Plasma,Tissue homogenate -
The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats
IF: 2.041Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019)PMID：31491333Reactivity：RatSample Type：Serum,Plasma,Tissue homogenate -
Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings
IF: 3.265 -
Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti?inflammatory effects, and inhibition of the p38?MAPK mechanism
IF: 1.554
实验操作视频
