Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
中文名称：大鼠低氧诱导因子1α(HIF-1α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠HIF-1α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠HIF-1α会与包被抗体结合。后依次加入生物素化的抗大鼠HIF-1α抗体和辣根过氧化物酶标记的亲和素，抗大鼠HIF-1α抗体与结合在包被抗体上的大鼠HIF-1α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，HIF-1α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中HIF-1α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠HIF-1α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Protective effect of rupatadine on testicular ischemia/reperfusion injury in rats: Modulation of IL-6/STAT3, Akt/ mTOR signaling pathways
IF: 3.300Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2024)Reactivity：RatSample Type：serum -
Butyrate-Mediated Modulation of Paraoxonase-1 Alleviates Cardiorenometabolic Abnormalities in a Rat Model of Polycystic Ovarian Syndrome
IF: 3.100Journal： CARDIOVASCULAR DRUGS AND THERAPY (2024)Reactivity：RatSample Type：cardiac tissue,renal tissue -
Sodium acetate prevents testicular damage in Wistar rats subjected to testicular ischaemia/reperfusion injury
IF: 2.800Journal： EXPERIMENTAL AND MOLECULAR PATHOLOGY (2024)Reactivity：RatSample Type：Testicular tissue -
Acetate ameliorates ovarian mitochondrial dysfunction in letrozole-induced polycystic ovarian syndrome rat model by improving mitofusin-2
IF: 2.600Journal： Journal of Physiological Sciences (2024)Reactivity：RatSample Type：ovarian tissue -
Acetate abates adipose-ovarian endocrinometabolic disturbance in experimentally induced polycystic ovarian syndrome
IF: 2.100Journal： STEROIDS (2024)Reactivity：RatSample Type：adipose tissue,ovarian tissue -
Acetate attenuates hypothalamic pyroptosis in experimentally induced polycystic ovarian syndrome
IF: 1.600Journal： BMC Research Notes (2024)Reactivity：RatSample Type：plasma,hypothalamus tissue -
Linagliptin counteracts rotenone’s toxicity in non-diabetic rat model of Parkinson’s disease: Insights into the neuroprotective roles of DJ-1, SIRT-1/Nrf-2 and implications of HIF1-α
IF: 5.000Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)PMID：36623635Reactivity：RatSample Type：midbrain,striatum -
Cardiac energy depletion in a rat model of polycystic ovarian syndrome is reversed by acetate and associated with inhibitory effect of HDAC2/mTOR
IF: 5.000 -
Anti-Arthritic Effect of Edaravone Against Complete Freund Adjuvant Induced Arthritis via Osteoclast Differentiation and HIF-1α–VEGF–ANG-1 Axis
IF: 4.800 -
Roxadustat ameliorates vascular calcification in CKD rats by regulating HIF-2α/HIF-1α
IF: 4.500
实验操作视频
